Three-dimensional construction of cultured cardiac myocytes and augmentation of their pulsatile ability by cell sheet manipulation utilizing temperature-responsive culture dishes

Tatsuya Shimizu; Masayuki Yamato; Akihiko Kikuchi; Teruo Okano

Three-dimensional construction of cultured cardiac myocytes and augmentation of their pulsatile ability by cell sheet manipulation utilizing temperature-responsive culture dishes

Tatsuya Shimizu, Masayuki Yamato, Akihiko Kikuchi, Teruo Okano

マテリアル創成工学科

研究成果: Paper › 査読

抄録

The utilization of temperature-responsive culture dishes for the construction of three-dimensional cultured cardiac myocytes was investigated. Primary chick cardiac myocytes were cultured on the substrates grafted with a temperature responsive polymer, poly(N-isopropylacrylamide) (PIPAAm), at 37°C, when substrates were slightly hydrophobic. Cross-sectional observation of the constructs revealed that three-dimensional cardiac tissues were fabricated by the unisotropic contraction and the fusion of cardiac myocyte sheets. The pulsatile ability of the cultured myocytes was augmented by the manipulation utilizing temperature-responsive culture dishes.

本文言語	English
ページ	75
ページ数	1
出版ステータス	Published - 2000
イベント	Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling - York, United Kingdom 継続期間: 16 7月 2000 → 19 7月 2000

Conference

Conference	Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling
国/地域	United Kingdom
City	York
Period	16/07/00 → 19/07/00

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引用スタイル

Shimizu, T., Yamato, M., Kikuchi, A., & Okano, T. (2000). Three-dimensional construction of cultured cardiac myocytes and augmentation of their pulsatile ability by cell sheet manipulation utilizing temperature-responsive culture dishes. 75. Paper presented at Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling, York, United Kingdom.

Shimizu, Tatsuya ; Yamato, Masayuki ; Kikuchi, Akihiko その他. / Three-dimensional construction of cultured cardiac myocytes and augmentation of their pulsatile ability by cell sheet manipulation utilizing temperature-responsive culture dishes. Paper presented at Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling, York, United Kingdom.1 p.

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abstract = "The utilization of temperature-responsive culture dishes for the construction of three-dimensional cultured cardiac myocytes was investigated. Primary chick cardiac myocytes were cultured on the substrates grafted with a temperature responsive polymer, poly(N-isopropylacrylamide) (PIPAAm), at 37°C, when substrates were slightly hydrophobic. Cross-sectional observation of the constructs revealed that three-dimensional cardiac tissues were fabricated by the unisotropic contraction and the fusion of cardiac myocyte sheets. The pulsatile ability of the cultured myocytes was augmented by the manipulation utilizing temperature-responsive culture dishes.",

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Shimizu, T, Yamato, M, Kikuchi, A & Okano, T 2000, 'Three-dimensional construction of cultured cardiac myocytes and augmentation of their pulsatile ability by cell sheet manipulation utilizing temperature-responsive culture dishes', Paper presented at Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling, York, United Kingdom, 16/07/00 - 19/07/00 pp. 75.

Three-dimensional construction of cultured cardiac myocytes and augmentation of their pulsatile ability by cell sheet manipulation utilizing temperature-responsive culture dishes. / Shimizu, Tatsuya; Yamato, Masayuki; Kikuchi, Akihiko その他.
2000. 75 Paper presented at Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling, York, United Kingdom.

研究成果: Paper › 査読

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Shimizu T, Yamato M, Kikuchi A, Okano T. Three-dimensional construction of cultured cardiac myocytes and augmentation of their pulsatile ability by cell sheet manipulation utilizing temperature-responsive culture dishes. 2000. Paper presented at Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling, York, United Kingdom.