TY - JOUR
T1 - Regulation of human FcεRI α-chain gene expression by multiple transcription factors
AU - Nishiyama, Chiharu
AU - Hasegawa, Masanari
AU - Nishiyama, Makoto
AU - Takahashi, Kyoko
AU - Akizawa, Yushiro
AU - Yokota, Toyokazu
AU - Okumura, Ko
AU - Ogawa, Hideoki
AU - Ra, Chisei
PY - 2002/5/1
Y1 - 2002/5/1
N2 - Transcriptional regulation of the gene-encoding human FcεRI α-chain was analyzed in detail. EMSA revealed that either YY1 or PU.1 bound to the region close to that recognized by Elf-1. The α-chain promoter activity was up-regulated ∼2-fold by exogenously expressed YY1 or PU.1 and ∼7-fold by GATA-1, respectively, in KU812 cells. In contrast, coexpression of GATA-1 with either of PU.1 or YY1 dramatically activated the promoter ∼41-or ∼27-fold, respectively. Especially synergic activation by GATA-1 and PU.1 was surprising, because these transcription factors are known to inhibit the respective transactivating activities of each other. These up-regulating effects of PU.1 and YY1 with GATA-1 were inhibited by overexpression of Elf-1, indicating that Elf-1 serves as a repressor for the α-chain gene expression. Transcriptional regulation of the α-chain gene through four transcriptional factors is discussed.
AB - Transcriptional regulation of the gene-encoding human FcεRI α-chain was analyzed in detail. EMSA revealed that either YY1 or PU.1 bound to the region close to that recognized by Elf-1. The α-chain promoter activity was up-regulated ∼2-fold by exogenously expressed YY1 or PU.1 and ∼7-fold by GATA-1, respectively, in KU812 cells. In contrast, coexpression of GATA-1 with either of PU.1 or YY1 dramatically activated the promoter ∼41-or ∼27-fold, respectively. Especially synergic activation by GATA-1 and PU.1 was surprising, because these transcription factors are known to inhibit the respective transactivating activities of each other. These up-regulating effects of PU.1 and YY1 with GATA-1 were inhibited by overexpression of Elf-1, indicating that Elf-1 serves as a repressor for the α-chain gene expression. Transcriptional regulation of the α-chain gene through four transcriptional factors is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0036569649&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.168.9.4546
DO - 10.4049/jimmunol.168.9.4546
M3 - Article
C2 - 11971001
AN - SCOPUS:0036569649
SN - 0022-1767
VL - 168
SP - 4546
EP - 4552
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -