Rational design of monodispersed mutants of proteins by identifying aggregation contact sites using solubilizing agents

Hiroaki Terasawa, Tatsuichiro Tsuji, Sosuke Yoshinaga, Mitsuhiro Takeda, Takafumi Sato, Akihiro Sonoda, Norihito Ishida, Kaori Yunoki, Etsuko Toda, Yuya Terashima, Kouji Matsushima

研究成果: Article査読

抄録

Suppression of protein aggregation is a subject of growing importance in the treatment of protein aggregation diseases, an urgent worldwide human health problem, and the production of therapeutic proteins, such as antibody drugs. We previously reported a method to identify compounds that suppress aggregation, based on screening using multiple terminal deletion mutants. We now present a method to determine the aggregation contact sites of proteins, using such solubilizing compounds, to design monodispersed mutants. We applied this strategy to the chemokine receptor-binding domain (CRBD) of FROUNT, which binds to the membrane-proximal C-terminal intracellular region of CCR2. Initially, the backbone NMR signals were assigned to a certain extent by available methods, and the putative locations of five α-helices were identified. Based on NMR chemical shift perturbations upon varying the protein concentrations, the first and third helices were found to contain the aggregation contact sites. The two helices are amphiphilic, and based on an NMR titration with 1,6- hexanediol, a CRBD solubilizing compound, the contact sites were identified as the hydrophobic patches located on the hydrophilic sides of the two helices. Subsequently, we designed multiple mutants targeting amino acid residues on the contact sites. Based on their NMR spectra, a doubly mutated CRBD (L538E/P612S) was selected from the designed mutants, and its monodispersed nature was confirmed by other biophysical methods. We then assessed the CCR2-binding activities of the mutants. Our method is useful for the protein structural analyses, the treatment of protein aggregation diseases, and the improvement of therapeutic proteins.

本文言語English
ページ(範囲)3639-3649
ページ数11
ジャーナルBiochemistry
59
39
DOI
出版ステータスPublished - 6 10 2020

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