TY - JOUR
T1 - Petroleum and polycyclic aromatic hydrocarbons (PAHs) degradation and naphthalene metabolism in Streptomyces sp. (ERI-CPDA-1) isolated from oil contaminated soil
AU - Balachandran, C.
AU - Duraipandiyan, V.
AU - Balakrishna, K.
AU - Ignacimuthu, S.
N1 - Funding Information:
The authors are grateful to Entomology Research Institute, Loyola College, Chennai , for financial assistance.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/5
Y1 - 2012/5
N2 - Petroleum and polycyclic aromatic hydrocarbons (PAHs) degrading . Streptomyces sp. isolate ERI-CPDA-1 was recovered from oil contaminated soil in Chennai, India. The degradation efficiencies were examined by GC-FID and the results showed that the isolate could remove 98.25% diesel oil, 99.14% naphthalene and 17.5% phenanthrene in 7. days at 30. °C (0.1%).ERI-CPDA-1 was able to degrade naphthalene, phenanthrene and diesel oil and grow on petrol, diesel, kerosene, benzene, pyridine, methanol, ethanol, cyclohexane, tween-80, xylene, DMSO and toluene using them as sole carbon source. Effects of environmental condition on degradation of hydrocarbons (diesel oil, naphthalene and phenanthrene) were also studied at different pH, NaCl, temperature, concentration and incubation time. Degradation pathway for naphthalene has been proposed. Degradation metabolites were identified using GC-MS analysis of ethyl acetate extract of the cell free culture. The degradation products detected were benzaldehyde, catechol, phenylacetic acid and protocatechuic acid.
AB - Petroleum and polycyclic aromatic hydrocarbons (PAHs) degrading . Streptomyces sp. isolate ERI-CPDA-1 was recovered from oil contaminated soil in Chennai, India. The degradation efficiencies were examined by GC-FID and the results showed that the isolate could remove 98.25% diesel oil, 99.14% naphthalene and 17.5% phenanthrene in 7. days at 30. °C (0.1%).ERI-CPDA-1 was able to degrade naphthalene, phenanthrene and diesel oil and grow on petrol, diesel, kerosene, benzene, pyridine, methanol, ethanol, cyclohexane, tween-80, xylene, DMSO and toluene using them as sole carbon source. Effects of environmental condition on degradation of hydrocarbons (diesel oil, naphthalene and phenanthrene) were also studied at different pH, NaCl, temperature, concentration and incubation time. Degradation pathway for naphthalene has been proposed. Degradation metabolites were identified using GC-MS analysis of ethyl acetate extract of the cell free culture. The degradation products detected were benzaldehyde, catechol, phenylacetic acid and protocatechuic acid.
KW - Biodegradation
KW - GC-MS
KW - Metabolic pathway
KW - Polycyclic aromatic hydrocarbons
KW - Streptomyces sp.
UR - http://www.scopus.com/inward/record.url?scp=84859211996&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2012.02.059
DO - 10.1016/j.biortech.2012.02.059
M3 - Article
C2 - 22425516
AN - SCOPUS:84859211996
SN - 0960-8524
VL - 112
SP - 83
EP - 90
JO - Bioresource Technology
JF - Bioresource Technology
ER -