Expressed protein ligation at methionine: N-terminal attachment of homocysteine, ligation, and masking

Tomohiro Tanaka, Anne M. Wagner, John B. Warner, Yanxin J. Wang, E. James Petersson

研究成果: Article査読

21 被引用数 (Scopus)

抄録

A useful handle: One major limitation of protein semi-synthesis is the need for Cys at the ligation site in native chemical ligation reactions. It is shown that a transferase enzyme can deliver homocysteine to the N-terminus of an expressed protein (see scheme). Homocysteine can be used in a ligation reaction and then converted to Met. This allows one to use the MetArg or MetLys motif as a point of disconnection in semi-synthesis.

本文言語English
ページ(範囲)6210-6213
ページ数4
ジャーナルAngewandte Chemie - International Edition
52
24
DOI
出版ステータスPublished - 10 6月 2013

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