Effect of polyols on membrane structures of liposomes: A study using small-angle X-ray scattering data and generalized indirect Fourier transformation

Aika Sekine, Taku Ogura, Koji Tsuchiya, Kaoru Ohishi, Yuji Masubuchi, Masaaki Akamatsu, Kenichi Sakai, Masahiko Abe, Hideki Sakai

研究成果: Article査読

抄録

This study aimed to evaluate the membrane structure of distearoylphosphatidylcholine (DSPC) liposomes dispersed in water containing various types of polyols with low molecular weight such as glycerin (Gly), 1,3-butandiol (BG), and propylene glycol (PG). To clarify the detailed membrane structure, generalized indirect Fourier transformation (GIFT) analysis, which provides information about the bilayer spacing, bilayer thickness, number of lamellar layers, and membrane flexibility, was applied to small-angle X-ray scattering (SAXS) data of the present system. The GIFT results showed that the bilayer thickness of the DSPC liposomes followed the order Gly>>BG>PG. In addition, the membrane flexibility estimated by the Caille parameter was in the order Gly>>BG>PG; this result was supported by the gel-liquid crystal phase transition temperature (Tc) obtained by differential scanning calorimetry (DSC). These results, together with the Raman spectra, suggest that BG and PG incorporated into the bilayers of DSPC liposomes result in the formation of an interdigitated lamellar structure.

本文言語English
論文番号105253
ジャーナルChemistry and Physics of Lipids
249
DOI
出版ステータスPublished - 11月 2022

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