TY - CONF
T1 - Control of cell detachment on poly(N-isopropylacrylamide) grafted glass sufaces using electron beam irradiation
AU - Fukumori, Kazuhiro
AU - Iwanaga, Shintaroh
AU - Akiyama, Yoshikatsu
AU - Kikuchi, Akihiko
AU - Yamato, Masayuki
AU - Sakai, Kiyotaka
AU - Okano, Teruo
PY - 2006
Y1 - 2006
N2 - PIPAAm was grafted onto coverslips modified with MPTMS, using EB irradiation. ESCA analysis of resulting surfaces revealed that PIPAAm was successfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. Cells adhered to the surfaces of 4.0 nm thickness of the grafted PIPAAm layer. By contrast, PIPAAm layer with 8.0 nm thickness exhibited cell repellent surfaces. This tendency is similar to PIPAAm grafted TCPS in our previous report, although the grafted layer is extremely thinner. We discuss an influence of thickness of the grafted PIPAAm onto cell adhesion / detachment properties, comparing to PIAPAm grafted TCPS in the previous report.
AB - PIPAAm was grafted onto coverslips modified with MPTMS, using EB irradiation. ESCA analysis of resulting surfaces revealed that PIPAAm was successfully grafted onto the coverslips. Initial monomer concentration significantly influenced properties of resulting PIPAAm grafted surfaces. Cells adhered to the surfaces of 4.0 nm thickness of the grafted PIPAAm layer. By contrast, PIPAAm layer with 8.0 nm thickness exhibited cell repellent surfaces. This tendency is similar to PIPAAm grafted TCPS in our previous report, although the grafted layer is extremely thinner. We discuss an influence of thickness of the grafted PIPAAm onto cell adhesion / detachment properties, comparing to PIAPAm grafted TCPS in the previous report.
KW - Electron beam irradiation
KW - N-isopropylacrylamide
KW - Thermoresponsive surface
KW - Total internal reflection microscopy
UR - http://www.scopus.com/inward/record.url?scp=33749860730&partnerID=8YFLogxK
M3 - Paper
AN - SCOPUS:33749860730
SP - 1977
T2 - 55th SPSJ Annual Meeting
Y2 - 24 May 2006 through 26 May 2006
ER -