Analyses of cell behavior onto micropatterned hydrophilic/hydrophobic surfaces

Introduction of capillary blood vessels is important to reconstruct complicated tissues and organs. We focused on microprocessing technique for possible construction of capillary blood vessels in vitro. In this study, hydrophilic poly(acrylamide)(PAAm) was immobilized at 100 μm ×100 μm with periodic lattice of 10 μm-width onto glass surfaces utilizing a modified liquid crystal display (LCD) projector and photolithography technique. PAAm pattern formation was confirmed by adsorption of FITC labeled BSA. Seeded endothelial cells adhered and extended only onto 10 μm-lattice glass domains but not onto hydrophilic PAAm domains, indicating successful and selective cell adhesion.