Unitary step of gliding machinery in Mycoplasma mobile

Yoshiaki Kinosita; Daisuke Nakane; Mitsuhiro Sugawa; Tomoko Masaike; Kana Mizutani; Makoto Miyata; Takayuki Nishizaka

doi:10.1073/pnas.1310355111

Unitary step of gliding machinery in Mycoplasma mobile

Yoshiaki Kinosita, Daisuke Nakane, Mitsuhiro Sugawa, Tomoko Masaike, Kana Mizutani, Makoto Miyata, Takayuki Nishizaka

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Among the bacteria that glide on substrate surfaces, Mycoplasma mobile is one of the fastest, exhibiting smooth movement with a speed of 2.0-4.5 μm·s^-1 with a cycle of attachment to and detachment from sialylated oligosaccharides. To study the gliding mechanism at the molecular level, we applied an assay with a fluorescently labeled and membrane- permeabilized ghost model, and investigated the motility by high precision colocalization microscopy. Under conditions designed to reduce the number of motor interactions on a randomly oriented substrate, ghosts took unitary 70-nm steps in the direction of gliding. Although it remains possible that the stepping behavior is produced by multiple interactions, our data suggest that these steps are produced by a unitary gliding machine that need not move between sites arranged on a cytoskeletal lattice.

Original language	English
Pages (from-to)	8601-8606
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	111
Issue number	23
DOIs	https://doi.org/10.1073/pnas.1310355111
Publication status	Published - 10 Jun 2014

Keywords

Bacteria tracking
Gliding ghost
Leg protein
Pairwise distance function

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10.1073/pnas.1310355111

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abstract = "Among the bacteria that glide on substrate surfaces, Mycoplasma mobile is one of the fastest, exhibiting smooth movement with a speed of 2.0-4.5 μm·s-1 with a cycle of attachment to and detachment from sialylated oligosaccharides. To study the gliding mechanism at the molecular level, we applied an assay with a fluorescently labeled and membrane- permeabilized ghost model, and investigated the motility by high precision colocalization microscopy. Under conditions designed to reduce the number of motor interactions on a randomly oriented substrate, ghosts took unitary 70-nm steps in the direction of gliding. Although it remains possible that the stepping behavior is produced by multiple interactions, our data suggest that these steps are produced by a unitary gliding machine that need not move between sites arranged on a cytoskeletal lattice.",

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AU - Kinosita, Yoshiaki

AU - Nakane, Daisuke

AU - Sugawa, Mitsuhiro

AU - Masaike, Tomoko

AU - Mizutani, Kana

AU - Miyata, Makoto

AU - Nishizaka, Takayuki

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KW - Leg protein

KW - Pairwise distance function

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Unitary step of gliding machinery in Mycoplasma mobile

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Keywords

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