TY - GEN
T1 - Two-dimensional multi-array formation of hepatocyte spheroids on a micro-fabricated PEG-brush surface
AU - Otsuka, H.
AU - Satomi, T.
AU - Itadani-Harada, J.
AU - Nagasaki, Y.
AU - Horiike, Y.
AU - Kataoka, K.
PY - 2004
Y1 - 2004
N2 - The micropatterning of rat primary hepatocyte hetero-spheroids was prepared on microfabricated glass substrates coated with poly(ethylene glycol) (PEG) brushes. Co-cultivation of hepatocytes and endothelial cells was used to stabilize hepatocyte viability and liver-specific functions. Rat primary hepatocytes, suspended in a culture medium, were applied to the patterned dishes with cultured endothelial cells selectively located in the circular domains. Rat primary hepatocytes formed spheroids within 24-h only on the circular regions of existing endothelial cells, generating a two-dimensional (2D)-arrayed structure of the hepatocyte spheroids.
AB - The micropatterning of rat primary hepatocyte hetero-spheroids was prepared on microfabricated glass substrates coated with poly(ethylene glycol) (PEG) brushes. Co-cultivation of hepatocytes and endothelial cells was used to stabilize hepatocyte viability and liver-specific functions. Rat primary hepatocytes, suspended in a culture medium, were applied to the patterned dishes with cultured endothelial cells selectively located in the circular domains. Rat primary hepatocytes formed spheroids within 24-h only on the circular regions of existing endothelial cells, generating a two-dimensional (2D)-arrayed structure of the hepatocyte spheroids.
UR - http://www.scopus.com/inward/record.url?scp=13844280863&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:13844280863
SN - 1877040193
SN - 9781877040191
T3 - Transactions - 7th World Biomaterials Congress
SP - 183
BT - Transactions - 7th World Biomaterials Congress
T2 - Transactions - 7th World Biomaterials Congress
Y2 - 17 May 2004 through 21 May 2004
ER -