Two-dimensional manipulation of confluent cultured cells

A. Kikuchi, M. Okuhara, F. Karikusa, H. Sakai, Y. Sakurai, T. Okano

Research output: Contribution to conferencePaperpeer-review

4 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide)-grafted (PIPAAm) dishes were utilized for the recovery of confluently cultured cells as a monolayer sheet by thermal treatment. The recovered cells were viable, keeping their morphologies as well as the cell-cell junctions. The density of hepatocytes (HC) recovered onto the bottom surface of the cell culture increased approximately 1.4 times than that observed on PIPAAm. The increase in cell density affected the function of recovered HC as judged by albumin synthesis as well as ornithine carbamoyltransferase (OCT) activity. Albumin synthesis increased after the recovery of HC onto the cell culture.

Original languageEnglish
Pages907
Number of pages1
Publication statusPublished - 1996
EventProceedings of the 1996 5th World Biomaterials Congress. Part 2 (of 2) - Toronto, Can
Duration: 29 May 19962 Jun 1996

Conference

ConferenceProceedings of the 1996 5th World Biomaterials Congress. Part 2 (of 2)
CityToronto, Can
Period29/05/962/06/96

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