TY - JOUR
T1 - Transmission electron microscopy observation of antibody
AU - Kamogawa, Marina
AU - Shimanuki, Junichi
AU - Azuma, Takachika
AU - Murakami, Akikazu
AU - Ishiguro, Takashi
N1 - Funding Information:
This work was supported by both a project of Center for Technologies against Cancer (2009-2013) and a project of Research Center for RNA Science (2010-2004) in Tokyo University of Science. These centers are funded by Program for Development of Strategic Research Center in Private Universities supported by the Ministry of Education, Culture, Sports, Science & Technology in Japan (MEXT).
PY - 2012
Y1 - 2012
N2 - High resolution electron microscopy (TEM) observation of an antibody, which is stained with sodium phosphotungstate solution, has been performed at accelerating voltage of 200 kV. In order to reduce background noise due to superposed substrate image, we observed antibodies standing on the edge of the mica substrate in the vacuum. Two typical images have been taken successfully. One shows Y-shaped contrast. The other image, which is thought to be a side view image, shows constricted part corresponding to hinge region. Both lengths of Fab (fragment, antigen binding) region and Fc (fagment, crystallizable) region are measured directly by referring lattice fringes of mica.
AB - High resolution electron microscopy (TEM) observation of an antibody, which is stained with sodium phosphotungstate solution, has been performed at accelerating voltage of 200 kV. In order to reduce background noise due to superposed substrate image, we observed antibodies standing on the edge of the mica substrate in the vacuum. Two typical images have been taken successfully. One shows Y-shaped contrast. The other image, which is thought to be a side view image, shows constricted part corresponding to hinge region. Both lengths of Fab (fragment, antigen binding) region and Fc (fagment, crystallizable) region are measured directly by referring lattice fringes of mica.
KW - Ionic liquid
KW - Mica
KW - Monoclonal antibody
KW - Sodium phosphotungstate
KW - Stain
KW - Transmission electron microscopy
UR - http://www.scopus.com/inward/record.url?scp=84892607015&partnerID=8YFLogxK
U2 - 10.1016/j.proeng.2012.03.023
DO - 10.1016/j.proeng.2012.03.023
M3 - Conference article
AN - SCOPUS:84892607015
SN - 1877-7058
VL - 36
SP - 150
EP - 153
JO - Procedia Engineering
JF - Procedia Engineering
T2 - 2011 IUMRS International Conference in Asia, ICA 2011
Y2 - 19 September 2011 through 22 September 2011
ER -