Transcriptional regulation of the human high affinity IgE receptor α-chain gene

Kyoko Takahashi; Chiharu Nishiyama; Chisei Ra

doi:10.1016/S0161-5890(02)00062-7

Transcriptional regulation of the human high affinity IgE receptor α-chain gene

Kyoko Takahashi, Chiharu Nishiyama, Chisei Ra

Department of Biological Science and Technology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Transcriptional regulation of the gene encoding human high affinity IgE receptor (FcεRI) α-chain was analyzed. Previously, we reported that GATA-1 and Elf-1 recognition sites were necessary for cell type-specific activation of the α-chain gene promoter. More detailed analysis revealed that other transcription factors bound the regions close to the Elf-1 recognition site and there was a more complex mechanism for the regulation of the promoter activity. On the other hand, during a course of studies to find cis-elements over this gene, CAGCTG sequence in the first intron was revealed to serve as an enhancer. A complex composed of USF1 and USF2 activated the human α-chain gene expression via this intronic element. Furthermore, we found two novel exons at 18.4 and 12.6kb upstream from the reported first exon and discovered an additional distal promoter.

Original language	English
Pages (from-to)	1193-1199
Number of pages	7
Journal	Molecular Immunology
Volume	38
Issue number	16-18
DOIs	https://doi.org/10.1016/S0161-5890(02)00062-7
Publication status	Published - 2002

Keywords

FcεRI
Transcription
α-chain

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10.1016/S0161-5890(02)00062-7

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title = "Transcriptional regulation of the human high affinity IgE receptor α-chain gene",

abstract = "Transcriptional regulation of the gene encoding human high affinity IgE receptor (FcεRI) α-chain was analyzed. Previously, we reported that GATA-1 and Elf-1 recognition sites were necessary for cell type-specific activation of the α-chain gene promoter. More detailed analysis revealed that other transcription factors bound the regions close to the Elf-1 recognition site and there was a more complex mechanism for the regulation of the promoter activity. On the other hand, during a course of studies to find cis-elements over this gene, CAGCTG sequence in the first intron was revealed to serve as an enhancer. A complex composed of USF1 and USF2 activated the human α-chain gene expression via this intronic element. Furthermore, we found two novel exons at 18.4 and 12.6kb upstream from the reported first exon and discovered an additional distal promoter.",

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AU - Nishiyama, Chiharu

AU - Ra, Chisei

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AB - Transcriptional regulation of the gene encoding human high affinity IgE receptor (FcεRI) α-chain was analyzed. Previously, we reported that GATA-1 and Elf-1 recognition sites were necessary for cell type-specific activation of the α-chain gene promoter. More detailed analysis revealed that other transcription factors bound the regions close to the Elf-1 recognition site and there was a more complex mechanism for the regulation of the promoter activity. On the other hand, during a course of studies to find cis-elements over this gene, CAGCTG sequence in the first intron was revealed to serve as an enhancer. A complex composed of USF1 and USF2 activated the human α-chain gene expression via this intronic element. Furthermore, we found two novel exons at 18.4 and 12.6kb upstream from the reported first exon and discovered an additional distal promoter.

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Transcriptional regulation of the human high affinity IgE receptor α-chain gene

Abstract

Keywords

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