Abstract
The utilization of temperature-responsive culture dishes for the construction of three-dimensional cultured cardiac myocytes was investigated. Primary chick cardiac myocytes were cultured on the substrates grafted with a temperature responsive polymer, poly(N-isopropylacrylamide) (PIPAAm), at 37°C, when substrates were slightly hydrophobic. Cross-sectional observation of the constructs revealed that three-dimensional cardiac tissues were fabricated by the unisotropic contraction and the fusion of cardiac myocyte sheets. The pulsatile ability of the cultured myocytes was augmented by the manipulation utilizing temperature-responsive culture dishes.
| Original language | English |
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| Pages | 75 |
| Number of pages | 1 |
| Publication status | Published - 2000 |
| Event | Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling - York, United Kingdom Duration: 16 Jul 2000 → 19 Jul 2000 |
Conference
| Conference | Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling |
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| Country/Territory | United Kingdom |
| City | York |
| Period | 16/07/00 → 19/07/00 |