Thermoresponsive patterned nanobio interfaces for application to patterned co-culture

Kenjiro Hira; Yukiko Tsuda; Akihiko Kikuchi; Kiyotaka Sakai; Teruo Okano

Thermoresponsive patterned nanobio interfaces for application to patterned co-culture

Kenjiro Hira, Yukiko Tsuda, Akihiko Kikuchi, Kiyotaka Sakai, Teruo Okano

Department of Materials Science and Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

In this study, photoinitiated iniferter polymerization was utilized to prepare thermoresponsive interfaces for possible application in tissue engineering. Poly(N-isopropylacrylamide) (PIPAAm) grafted layers were introduced onto glass surfaces by iniferter polymerization method. Surface analyses including static contact angle measurements, XPS analyses and AFM revealed that the surfaces were successfully modified with PIPAAm. PIPAAm-grafted surfaces showed cell adhesion/detachment control by changing initial polymerization conditions. By using porous metal mask, PIPAAm-patterned surfaces were obtained. Cells only adhered onto PIPAAm ungrafted domains at 27°C and these adhered cells proliferated to reach confluency at 37°C. These patterned thermoresponsive surfaces should be utilized to prepare well-defined patterned co-culture cell sheets valuable for tissue regenerations.

Original language	English
Number of pages	1
Publication status	Published - 19 Oct 2006
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 24 May 2006 → 26 May 2006

Conference

Conference	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	24/05/06 → 26/05/06

Keywords

Endotherial cells
Iniferter polymerization
Poly(N-isopropylacryamide)
Thermoresponsive surfaces

Cite this

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title = "Thermoresponsive patterned nanobio interfaces for application to patterned co-culture",

abstract = "In this study, photoinitiated iniferter polymerization was utilized to prepare thermoresponsive interfaces for possible application in tissue engineering. Poly(N-isopropylacrylamide) (PIPAAm) grafted layers were introduced onto glass surfaces by iniferter polymerization method. Surface analyses including static contact angle measurements, XPS analyses and AFM revealed that the surfaces were successfully modified with PIPAAm. PIPAAm-grafted surfaces showed cell adhesion/detachment control by changing initial polymerization conditions. By using porous metal mask, PIPAAm-patterned surfaces were obtained. Cells only adhered onto PIPAAm ungrafted domains at 27°C and these adhered cells proliferated to reach confluency at 37°C. These patterned thermoresponsive surfaces should be utilized to prepare well-defined patterned co-culture cell sheets valuable for tissue regenerations.",

keywords = "Endotherial cells, Iniferter polymerization, Poly(N-isopropylacryamide), Thermoresponsive surfaces",

author = "Kenjiro Hira and Yukiko Tsuda and Akihiko Kikuchi and Kiyotaka Sakai and Teruo Okano",

year = "2006",

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TY - CONF

T1 - Thermoresponsive patterned nanobio interfaces for application to patterned co-culture

AU - Hira, Kenjiro

AU - Tsuda, Yukiko

AU - Kikuchi, Akihiko

AU - Sakai, Kiyotaka

AU - Okano, Teruo

PY - 2006/10/19

Y1 - 2006/10/19

N2 - In this study, photoinitiated iniferter polymerization was utilized to prepare thermoresponsive interfaces for possible application in tissue engineering. Poly(N-isopropylacrylamide) (PIPAAm) grafted layers were introduced onto glass surfaces by iniferter polymerization method. Surface analyses including static contact angle measurements, XPS analyses and AFM revealed that the surfaces were successfully modified with PIPAAm. PIPAAm-grafted surfaces showed cell adhesion/detachment control by changing initial polymerization conditions. By using porous metal mask, PIPAAm-patterned surfaces were obtained. Cells only adhered onto PIPAAm ungrafted domains at 27°C and these adhered cells proliferated to reach confluency at 37°C. These patterned thermoresponsive surfaces should be utilized to prepare well-defined patterned co-culture cell sheets valuable for tissue regenerations.

AB - In this study, photoinitiated iniferter polymerization was utilized to prepare thermoresponsive interfaces for possible application in tissue engineering. Poly(N-isopropylacrylamide) (PIPAAm) grafted layers were introduced onto glass surfaces by iniferter polymerization method. Surface analyses including static contact angle measurements, XPS analyses and AFM revealed that the surfaces were successfully modified with PIPAAm. PIPAAm-grafted surfaces showed cell adhesion/detachment control by changing initial polymerization conditions. By using porous metal mask, PIPAAm-patterned surfaces were obtained. Cells only adhered onto PIPAAm ungrafted domains at 27°C and these adhered cells proliferated to reach confluency at 37°C. These patterned thermoresponsive surfaces should be utilized to prepare well-defined patterned co-culture cell sheets valuable for tissue regenerations.

KW - Endotherial cells

KW - Iniferter polymerization

KW - Poly(N-isopropylacryamide)

KW - Thermoresponsive surfaces

UR - http://www.scopus.com/inward/record.url?scp=33749858105&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33749858105

T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

ER -

Thermoresponsive patterned nanobio interfaces for application to patterned co-culture

Abstract

Conference

Keywords

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