Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment

Aya Mizutani; Akihiko Kikuchi; Jun Kobayashi; Yoshikatsu Akiyama; Hideko Kanazawa; Teruo Okano

Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment

Aya Mizutani, Akihiko Kikuchi, Jun Kobayashi, Yoshikatsu Akiyama, Hideko Kanazawa, Teruo Okano

Department of Materials Science and Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

In the present research, thermoresponsive interfaces grafted with high-density poly(N-isopropylacrylamide) (PIPAAm) are prepared by surface-initiated atom transfer radical polymerization (ATRP) on poly(p-chloromethylstyrene)-cast polystyrene surfaces. Surface characterization revealed that thermoresponsive polymers were covalently grafted onto the polystyrene surfaces. Moreover, we investigated influence of thermoresponsive surface characteristics on adhesion/detachment behavior of cultured cells. Results suggest that control of the amount of grafted PIPAAm, and also the differences in its PIPAAm constructed structure influence cell adhesion/detachment control.

Original language	English
Pages	5399-5400
Number of pages	2
Publication status	Published - 1 Dec 2006
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: 20 Sept 2006 → 22 Sept 2006

Conference

Conference	55th Society of Polymer Science Japan Symposium on Macromolecules
Country/Territory	Japan
City	Toyama
Period	20/09/06 → 22/09/06

Keywords

Cell culture
High-density polymer grafted surfaces
Poly(N-isopropylacrylamide)
Surface-initiated atom transfer radical polymerization
Thermoresponsive polymer

Cite this

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title = "Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment",

abstract = "In the present research, thermoresponsive interfaces grafted with high-density poly(N-isopropylacrylamide) (PIPAAm) are prepared by surface-initiated atom transfer radical polymerization (ATRP) on poly(p-chloromethylstyrene)-cast polystyrene surfaces. Surface characterization revealed that thermoresponsive polymers were covalently grafted onto the polystyrene surfaces. Moreover, we investigated influence of thermoresponsive surface characteristics on adhesion/detachment behavior of cultured cells. Results suggest that control of the amount of grafted PIPAAm, and also the differences in its PIPAAm constructed structure influence cell adhesion/detachment control.",

keywords = "Cell culture, High-density polymer grafted surfaces, Poly(N-isopropylacrylamide), Surface-initiated atom transfer radical polymerization, Thermoresponsive polymer",

author = "Aya Mizutani and Akihiko Kikuchi and Jun Kobayashi and Yoshikatsu Akiyama and Hideko Kanazawa and Teruo Okano",

year = "2006",

month = dec,

day = "1",

language = "English",

pages = "5399--5400",

note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

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Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment. / Mizutani, Aya; Kikuchi, Akihiko; Kobayashi, Jun et al.
2006. 5399-5400 Paper presented at 55th Society of Polymer Science Japan Symposium on Macromolecules, Toyama, Japan.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment

AU - Mizutani, Aya

AU - Kikuchi, Akihiko

AU - Kobayashi, Jun

AU - Akiyama, Yoshikatsu

AU - Kanazawa, Hideko

AU - Okano, Teruo

PY - 2006/12/1

Y1 - 2006/12/1

N2 - In the present research, thermoresponsive interfaces grafted with high-density poly(N-isopropylacrylamide) (PIPAAm) are prepared by surface-initiated atom transfer radical polymerization (ATRP) on poly(p-chloromethylstyrene)-cast polystyrene surfaces. Surface characterization revealed that thermoresponsive polymers were covalently grafted onto the polystyrene surfaces. Moreover, we investigated influence of thermoresponsive surface characteristics on adhesion/detachment behavior of cultured cells. Results suggest that control of the amount of grafted PIPAAm, and also the differences in its PIPAAm constructed structure influence cell adhesion/detachment control.

AB - In the present research, thermoresponsive interfaces grafted with high-density poly(N-isopropylacrylamide) (PIPAAm) are prepared by surface-initiated atom transfer radical polymerization (ATRP) on poly(p-chloromethylstyrene)-cast polystyrene surfaces. Surface characterization revealed that thermoresponsive polymers were covalently grafted onto the polystyrene surfaces. Moreover, we investigated influence of thermoresponsive surface characteristics on adhesion/detachment behavior of cultured cells. Results suggest that control of the amount of grafted PIPAAm, and also the differences in its PIPAAm constructed structure influence cell adhesion/detachment control.

KW - Cell culture

KW - High-density polymer grafted surfaces

KW - Poly(N-isopropylacrylamide)

KW - Surface-initiated atom transfer radical polymerization

KW - Thermoresponsive polymer

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M3 - Paper

AN - SCOPUS:33846161290

SP - 5399

EP - 5400

T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Thermoresponsive interfaces grafted with high-density thermoresponsive polymers; Regulated cultured cell adhesion and detachment

Abstract

Conference

Keywords

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