Abstract
We have developed a novel strategy for regenerative medicine to recover tissue functions by using temperature-responsive cell culture surfaces. To overcome the limits of conventional methods such as the use of single-cell suspension injection and the use of biodegradable polymer scaffolds, we have applied transplantable cell sheets fabricated with temperature-responsive culture surfaces for cell delivery. Here, we summarize the development of the next generation of temperature-responsive cell culture surfaces using modifications of the polymer coating to allow for rapid cell sheet recovery; improved culture conditions, such that animal-derived products can be eliminated; and altered phase transition temperatures to produce patterned cell sheets. These designs allow for the reconstruction of more complex tissues to potentially treat a wide range of diseases.
| Original language | English |
|---|---|
| Pages (from-to) | 1123-1133 |
| Number of pages | 11 |
| Journal | Progress in Polymer Science (Oxford) |
| Volume | 32 |
| Issue number | 8-9 |
| DOIs | |
| Publication status | Published - Aug 2007 |
Keywords
- Cell sheet engineering
- Cell-based therapy
- Porous membrane
- Temperature-responsive culture dishes