Surface plasmon resonance (SPR) study on the relationship between biorecognition of lactosyl PEGylated gold nanoparticle against RCA120 lectin and the chain length of the PEG strands

Takao Jinbo; Seiji Takae; Yoshitsugu Akiyama; Hidenori Otsuka; Yuichi Yamasaki; Kazunori Kataoka

Surface plasmon resonance (SPR) study on the relationship between biorecognition of lactosyl PEGylated gold nanoparticle against RCA₁₂₀ lectin and the chain length of the PEG strands

Takao Jinbo, Seiji Takae, Yoshitsugu Akiyama, Hidenori Otsuka, Yuichi Yamasaki, Kazunori Kataoka

Research output: Contribution to conference › Paper › peer-review

Abstract

To achieve in vivo active targeting of colloidal carriers, the introduction of ligand on the surface of the carrier was quite promising. In this study, as a model of ligand-installed colloidal carriers, lactosyl-PEGylated gold nanoparticles were employed. For the preparation of the nanoparticles, lactose-functionalized PEG disulfide dimer, (lactose-PEG-S)₂, was newly synthesized through reductive amination. Then, lactosyl-PEGylated gold nanoparticles were prepared by the addition of gold nanoparticles to the mixture of the (lactose-PEG-S)₂ and non-lactosyl-polymer, (PEG-S) ₂, possessing various chain length. The binding amount of the nanoparticles against lectin-immobilized sensorchip was evaluated with SPR.

Original language	English
Pages	5006
Number of pages	1
Publication status	Published - 2005
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 20 Sept 2005 → 22 Sept 2005

Conference

Conference	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	20/09/05 → 22/09/05

Keywords

Lactose
Lectin
Length of poly (ethylene glycol)
Poly (ethylene glycol)
Surface plasmon resonance (SPR)

Cite this

@conference{f5d987859e8f4601a7a36d809a41ac5a,

title = "Surface plasmon resonance (SPR) study on the relationship between biorecognition of lactosyl PEGylated gold nanoparticle against RCA120 lectin and the chain length of the PEG strands",

abstract = "To achieve in vivo active targeting of colloidal carriers, the introduction of ligand on the surface of the carrier was quite promising. In this study, as a model of ligand-installed colloidal carriers, lactosyl-PEGylated gold nanoparticles were employed. For the preparation of the nanoparticles, lactose-functionalized PEG disulfide dimer, (lactose-PEG-S)2, was newly synthesized through reductive amination. Then, lactosyl-PEGylated gold nanoparticles were prepared by the addition of gold nanoparticles to the mixture of the (lactose-PEG-S)2 and non-lactosyl-polymer, (PEG-S) 2, possessing various chain length. The binding amount of the nanoparticles against lectin-immobilized sensorchip was evaluated with SPR.",

keywords = "Lactose, Lectin, Length of poly (ethylene glycol), Poly (ethylene glycol), Surface plasmon resonance (SPR)",

author = "Takao Jinbo and Seiji Takae and Yoshitsugu Akiyama and Hidenori Otsuka and Yuichi Yamasaki and Kazunori Kataoka",

year = "2005",

language = "English",

pages = "5006",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

}

Surface plasmon resonance (SPR) study on the relationship between biorecognition of lactosyl PEGylated gold nanoparticle against RCA₁₂₀ lectin and the chain length of the PEG strands. / Jinbo, Takao; Takae, Seiji; Akiyama, Yoshitsugu et al.
2005. 5006 Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Surface plasmon resonance (SPR) study on the relationship between biorecognition of lactosyl PEGylated gold nanoparticle against RCA120 lectin and the chain length of the PEG strands

AU - Jinbo, Takao

AU - Takae, Seiji

AU - Akiyama, Yoshitsugu

AU - Otsuka, Hidenori

AU - Yamasaki, Yuichi

AU - Kataoka, Kazunori

PY - 2005

Y1 - 2005

N2 - To achieve in vivo active targeting of colloidal carriers, the introduction of ligand on the surface of the carrier was quite promising. In this study, as a model of ligand-installed colloidal carriers, lactosyl-PEGylated gold nanoparticles were employed. For the preparation of the nanoparticles, lactose-functionalized PEG disulfide dimer, (lactose-PEG-S)2, was newly synthesized through reductive amination. Then, lactosyl-PEGylated gold nanoparticles were prepared by the addition of gold nanoparticles to the mixture of the (lactose-PEG-S)2 and non-lactosyl-polymer, (PEG-S) 2, possessing various chain length. The binding amount of the nanoparticles against lectin-immobilized sensorchip was evaluated with SPR.

AB - To achieve in vivo active targeting of colloidal carriers, the introduction of ligand on the surface of the carrier was quite promising. In this study, as a model of ligand-installed colloidal carriers, lactosyl-PEGylated gold nanoparticles were employed. For the preparation of the nanoparticles, lactose-functionalized PEG disulfide dimer, (lactose-PEG-S)2, was newly synthesized through reductive amination. Then, lactosyl-PEGylated gold nanoparticles were prepared by the addition of gold nanoparticles to the mixture of the (lactose-PEG-S)2 and non-lactosyl-polymer, (PEG-S) 2, possessing various chain length. The binding amount of the nanoparticles against lectin-immobilized sensorchip was evaluated with SPR.

KW - Lactose

KW - Lectin

KW - Length of poly (ethylene glycol)

KW - Poly (ethylene glycol)

KW - Surface plasmon resonance (SPR)

UR - http://www.scopus.com/inward/record.url?scp=33645642562&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645642562

SP - 5006

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Surface plasmon resonance (SPR) study on the relationship between biorecognition of lactosyl PEGylated gold nanoparticle against RCA₁₂₀ lectin and the chain length of the PEG strands

Abstract

Conference

Keywords

Other files and links

Fingerprint

Cite this