TY - GEN
T1 - Surface plasmon resonance biosensing by heterobifunctional poly (ethylene glycol) tethered chain surface
AU - Uchida, K.
AU - Ishii, T.
AU - Nagasaki, Y.
AU - Kato, M.
AU - Takahashi, T.
AU - Otsuka, Hidenori
AU - Kataoka, K.
PY - 2004/12/1
Y1 - 2004/12/1
N2 - A novel surface plasmon resonance (SPR) gold sensor chip that had heterobifunctional poly(ethylene glycol) (hetero-PEG) was proposed. The non-specific adsorption and specific recognition of a biomolecule that interacted with a ligand molecule installed at the free-end of the hetero-PEG chain were also discussed. The non-specific protein adsorption on PEGylated sensor surfaces was evaluated using BSA protein. Despite a low concentration of streptavidin, SPR profile on biotinylated PEG surface for streptavidin changed significantly. It was suggested that, PEG surface possessing a ligand at PEG chain end exhibited high sensing ability.
AB - A novel surface plasmon resonance (SPR) gold sensor chip that had heterobifunctional poly(ethylene glycol) (hetero-PEG) was proposed. The non-specific adsorption and specific recognition of a biomolecule that interacted with a ligand molecule installed at the free-end of the hetero-PEG chain were also discussed. The non-specific protein adsorption on PEGylated sensor surfaces was evaluated using BSA protein. Despite a low concentration of streptavidin, SPR profile on biotinylated PEG surface for streptavidin changed significantly. It was suggested that, PEG surface possessing a ligand at PEG chain end exhibited high sensing ability.
UR - http://www.scopus.com/inward/record.url?scp=13844298913&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:13844298913
SN - 1877040193
SN - 9781877040191
T3 - Transactions - 7th World Biomaterials Congress
BT - Transactions - 7th World Biomaterials Congress
T2 - Transactions - 7th World Biomaterials Congress
Y2 - 17 May 2004 through 21 May 2004
ER -