Abstract
A sensitive and stable porous carbon-modified enzyme sensor based on mediated electron transfer (MET) was developed for d-glucose detection. Toluidine blue (TB) was loaded as a mediator into the pores of porous carbon particles, and the pores were capped by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) by one-step drop-casting of TB and FAD-GDH. TB was found to be stably capped in the pores by FAD-GDH for 21 d. The resulting sensor enabled the reproducible and sensitive detection of d-glucose with a detection limit of 6.5 µM. The present sensor is promising for the long-term monitoring of low concentrations of biomarkers.
| Original language | English |
|---|---|
| Pages (from-to) | 3245-3253 |
| Number of pages | 9 |
| Journal | Sensors and Materials |
| Volume | 33 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2021 |
Keywords
- Biosensor
- Capping technique
- Electrochemistry
- Enzyme
- Porous carbon
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