Stable immobilization of mediator in porous carbon material via capping technique for fabricating sensitive enzyme-based glucose sensor

Kuniaki Nagamine; Ryota Izawa; Isao Shitanda; Shizuo Tokito

doi:10.18494/SAM.2021.3583

Stable immobilization of mediator in porous carbon material via capping technique for fabricating sensitive enzyme-based glucose sensor

Kuniaki Nagamine, Ryota Izawa, Isao Shitanda, Shizuo Tokito

Department of Pure and Applied Chemistry

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A sensitive and stable porous carbon-modified enzyme sensor based on mediated electron transfer (MET) was developed for d-glucose detection. Toluidine blue (TB) was loaded as a mediator into the pores of porous carbon particles, and the pores were capped by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) by one-step drop-casting of TB and FAD-GDH. TB was found to be stably capped in the pores by FAD-GDH for 21 d. The resulting sensor enabled the reproducible and sensitive detection of d-glucose with a detection limit of 6.5 µM. The present sensor is promising for the long-term monitoring of low concentrations of biomarkers.

Original language	English
Pages (from-to)	3245-3253
Number of pages	9
Journal	Sensors and Materials
Volume	33
Issue number	9
DOIs	https://doi.org/10.18494/SAM.2021.3583
Publication status	Published - 2021

Keywords

Biosensor
Capping technique
Electrochemistry
Enzyme
Porous carbon

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abstract = "A sensitive and stable porous carbon-modified enzyme sensor based on mediated electron transfer (MET) was developed for d-glucose detection. Toluidine blue (TB) was loaded as a mediator into the pores of porous carbon particles, and the pores were capped by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) by one-step drop-casting of TB and FAD-GDH. TB was found to be stably capped in the pores by FAD-GDH for 21 d. The resulting sensor enabled the reproducible and sensitive detection of d-glucose with a detection limit of 6.5 µM. The present sensor is promising for the long-term monitoring of low concentrations of biomarkers.",

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AU - Tokito, Shizuo

PY - 2021

Y1 - 2021

N2 - A sensitive and stable porous carbon-modified enzyme sensor based on mediated electron transfer (MET) was developed for d-glucose detection. Toluidine blue (TB) was loaded as a mediator into the pores of porous carbon particles, and the pores were capped by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) by one-step drop-casting of TB and FAD-GDH. TB was found to be stably capped in the pores by FAD-GDH for 21 d. The resulting sensor enabled the reproducible and sensitive detection of d-glucose with a detection limit of 6.5 µM. The present sensor is promising for the long-term monitoring of low concentrations of biomarkers.

AB - A sensitive and stable porous carbon-modified enzyme sensor based on mediated electron transfer (MET) was developed for d-glucose detection. Toluidine blue (TB) was loaded as a mediator into the pores of porous carbon particles, and the pores were capped by flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) by one-step drop-casting of TB and FAD-GDH. TB was found to be stably capped in the pores by FAD-GDH for 21 d. The resulting sensor enabled the reproducible and sensitive detection of d-glucose with a detection limit of 6.5 µM. The present sensor is promising for the long-term monitoring of low concentrations of biomarkers.

KW - Biosensor

KW - Capping technique

KW - Electrochemistry

KW - Enzyme

KW - Porous carbon

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JO - Sensors and Materials

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Stable immobilization of mediator in porous carbon material via capping technique for fabricating sensitive enzyme-based glucose sensor

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Keywords

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