Splice isoforms of transcription factor elf-1 affecting its regulatory function in transcription—molecular cloning of rat elf-1

Chiharu Nishiyama; Kyoko Takahashi; Makoto Nishiyama; Ko Okumura; Chisei Ra; Yasuyuki Ohtake; Toyokazu Yokota

doi:10.1271/bbb.64.2601

Splice isoforms of transcription factor elf-1 affecting its regulatory function in transcription—molecular cloning of rat elf-1

Chiharu Nishiyama, Kyoko Takahashi, Makoto Nishiyama, Ko Okumura, Chisei Ra, Yasuyuki Ohtake, Toyokazu Yokota

Department of Biological Science and Technology

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

To elucidate the role of Elf-1 in FcεRI α chain expression, rat Elf-1 cDNAs were isolated and characterized. The rat Elf-1 cDNA of 2744 bp contained an open reading frame of 1848 bp. In addition to the full length rat Elf-1 cDNA (named type 1), two splice isoforms were isolated. One of the two isoforms lacked the amino acid residues from 85th to 120th (type 2), and the other from 85th to 175th (type 3). Similar isoforms were also observed in human tissue. Overexpression of rat Elf-1 (type 1) using a transient coexpression system inhibited of the α chain promoter activity. The inhibition activity was different between the isoforms; the inhibition activity of type 2 was lower than that of type 1, and type 3 did not have an inhibitory effect. This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control.

Original language	English
Pages (from-to)	2601-2607
Number of pages	7
Journal	Bioscience, Biotechnology and Biochemistry
Volume	64
Issue number	12
DOIs	https://doi.org/10.1271/bbb.64.2601
Publication status	Published - Jun 2000

Keywords

Cdna
Elf-1
FcεRI
Splice isoform

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title = "Splice isoforms of transcription factor elf-1 affecting its regulatory function in transcription—molecular cloning of rat elf-1",

abstract = "To elucidate the role of Elf-1 in FcεRI α chain expression, rat Elf-1 cDNAs were isolated and characterized. The rat Elf-1 cDNA of 2744 bp contained an open reading frame of 1848 bp. In addition to the full length rat Elf-1 cDNA (named type 1), two splice isoforms were isolated. One of the two isoforms lacked the amino acid residues from 85th to 120th (type 2), and the other from 85th to 175th (type 3). Similar isoforms were also observed in human tissue. Overexpression of rat Elf-1 (type 1) using a transient coexpression system inhibited of the α chain promoter activity. The inhibition activity was different between the isoforms; the inhibition activity of type 2 was lower than that of type 1, and type 3 did not have an inhibitory effect. This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control.",

keywords = "Cdna, Elf-1, FcεRI, Splice isoform",

author = "Chiharu Nishiyama and Kyoko Takahashi and Makoto Nishiyama and Ko Okumura and Chisei Ra and Yasuyuki Ohtake and Toyokazu Yokota",

year = "2000",

month = jun,

doi = "10.1271/bbb.64.2601",

language = "English",

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pages = "2601--2607",

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T1 - Splice isoforms of transcription factor elf-1 affecting its regulatory function in transcription—molecular cloning of rat elf-1

AU - Nishiyama, Chiharu

AU - Takahashi, Kyoko

AU - Nishiyama, Makoto

AU - Okumura, Ko

AU - Ra, Chisei

AU - Ohtake, Yasuyuki

AU - Yokota, Toyokazu

PY - 2000/6

Y1 - 2000/6

N2 - To elucidate the role of Elf-1 in FcεRI α chain expression, rat Elf-1 cDNAs were isolated and characterized. The rat Elf-1 cDNA of 2744 bp contained an open reading frame of 1848 bp. In addition to the full length rat Elf-1 cDNA (named type 1), two splice isoforms were isolated. One of the two isoforms lacked the amino acid residues from 85th to 120th (type 2), and the other from 85th to 175th (type 3). Similar isoforms were also observed in human tissue. Overexpression of rat Elf-1 (type 1) using a transient coexpression system inhibited of the α chain promoter activity. The inhibition activity was different between the isoforms; the inhibition activity of type 2 was lower than that of type 1, and type 3 did not have an inhibitory effect. This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control.

AB - To elucidate the role of Elf-1 in FcεRI α chain expression, rat Elf-1 cDNAs were isolated and characterized. The rat Elf-1 cDNA of 2744 bp contained an open reading frame of 1848 bp. In addition to the full length rat Elf-1 cDNA (named type 1), two splice isoforms were isolated. One of the two isoforms lacked the amino acid residues from 85th to 120th (type 2), and the other from 85th to 175th (type 3). Similar isoforms were also observed in human tissue. Overexpression of rat Elf-1 (type 1) using a transient coexpression system inhibited of the α chain promoter activity. The inhibition activity was different between the isoforms; the inhibition activity of type 2 was lower than that of type 1, and type 3 did not have an inhibitory effect. This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control.

KW - Cdna

KW - Elf-1

KW - FcεRI

KW - Splice isoform

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AN - SCOPUS:0034567581

SN - 0916-8451

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 12

ER -

Splice isoforms of transcription factor elf-1 affecting its regulatory function in transcription—molecular cloning of rat elf-1

Abstract

Keywords

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