Sonoporation in developmental biology

Sho Ohta, Kentaro Suzuki, Shinichi Miyagawa, Yukiko Ogino, Mylah Villacorte, Yoshihiro Wada, Gen Yamada

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Recent, molecular biology techniques have accomplished a large contribution to developmental biology. Especially, gene transduction techniques are indispensable to study the roles of regulatory genes underlying embryogenesis. Replication-competent retroviruses, transfection with lipofection and an in ovo electroporation have been established as gene transduction techniques for embryos. (Yamada et al., 1997; Muramatsu et al., 1998; Fukuda et al., 2000; Iba, 2000; Nakamura et al., 2000). Particularly, in ovo electroporation for chick embryos has been recognized as a powerful method to efficiently induce exogenous genes into target cells or tissues, and it has been widely utilized to investigate their functions during embryonic development. Because the electric current tend to affect mainly the epithelial cells of the embryo, in ovo electroporation is suitable for the studies of neurogenesis and neuronal differentiation. In fact, large number of information relating to neuronal development has been brought from the studies using in ovo electroporation (Okafuji et al., 1999; Nakamura and Funahashi, 2001).

Original languageEnglish
Title of host publicationElectroporation and Sonoporation in Developmental Biology
PublisherSpringer Japan
Pages317-326
Number of pages10
ISBN (Print)9784431094265
DOIs
Publication statusPublished - 1 Dec 2009

Cite this

Ohta, S., Suzuki, K., Miyagawa, S., Ogino, Y., Villacorte, M., Wada, Y., & Yamada, G. (2009). Sonoporation in developmental biology. In Electroporation and Sonoporation in Developmental Biology (pp. 317-326). Springer Japan. https://doi.org/10.1007/978-4-431-09427-2_27