Single Nucleotide Polymorphisms of HAAO and IRX6 Genes as Risk Factors for Hypospadias

Yoshiyuki Kojima, Tomoyuki Koguchi, Kentaro Mizuno, Yuichi Sato, Seiji Hoshi, Junya Hata, Hidenori Nishio, Daiki Hashimoto, Shoko Matsushita, Kentaro Suzuki, Shinichi Miyagawa, Chi Chung Hui, Chizu Tanikawa, Yoshimori Murakami, Gen Yamada, Yutaro Hayashi, Koichi Matsuda

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Abstract

Purpose:We evaluated the association of hypospadias and 17 susceptibility loci previously identified by a European genome-wide association study in a cohort of Japanese patients. We also examined the expression of candidate genes in male mouse embryos to determine the possible underlying mechanisms of this disease.Materials and Methods:We enrolled 169 Japanese patients (mean age at surgery 3.7 years) who underwent repair of hypospadias. Genotyping of 17 single nucleotide polymorphisms was performed using a multiplex polymerase chain reaction invader assay. We also performed in situ hybridization to determine whether candidate genes were expressed in the male genital tubercle during embryonic development of the external genitalia in mice.Results:Single nucleotide polymorphism rs3816183 of HAAO was significantly associated with susceptibility to hypospadias in general (p = 0.0019) and to anterior/middle hypospadias (p = 0.0283) and posterior hypospadias (p = 0.0226), while single nucleotide polymorphism rs6499755 of IRX6 showed an association with susceptibility to anterior/middle hypospadias (p = 0.0472). In mouse embryos there was no significant upregulation of Haao expression in the developing male external genitalia. Irx3 and Irx5, which are linked to Irx6 within the IrxB cluster, were expressed in the mesenchyme remote from the urethral plate epithelium during the critical embryonic period for masculinization. Irx6 was expressed in the ectodermal epithelium, demonstrating prominent dorsal ectodermal expression without expression in the ventral ectoderm adjacent to the urethral plate during the same period.Conclusions:Genetic variations of HAAO and IRX6 influence susceptibility to hypospadias in the Japanese population. Further research is needed to clarify the mechanism by which variations in these genes contribute to the pathogenesis of hypospadias.

Original languageEnglish
Pages (from-to)386-392
Number of pages7
JournalJournal of Urology
Volume201
Issue number2
DOIs
Publication statusPublished - 1 Feb 2019

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Keywords

  • hypospadias
  • in situ hybridization
  • polymorphism,
  • single nucleotide

Cite this

Kojima, Y., Koguchi, T., Mizuno, K., Sato, Y., Hoshi, S., Hata, J., Nishio, H., Hashimoto, D., Matsushita, S., Suzuki, K., Miyagawa, S., Hui, C. C., Tanikawa, C., Murakami, Y., Yamada, G., Hayashi, Y., & Matsuda, K. (2019). Single Nucleotide Polymorphisms of HAAO and IRX6 Genes as Risk Factors for Hypospadias. Journal of Urology, 201(2), 386-392. https://doi.org/10.1016/j.juro.2018.07.050