TY - JOUR
T1 - Sensitive electrochemical detection of L-Cysteine at a screen-printed diamond electrode
AU - Matsunaga, Tomohiro
AU - Kondo, Takeshi
AU - Shitanda, Isao
AU - Hoshi, Yoshinao
AU - Itagaki, Masayuki
AU - Tojo, Toshifumi
AU - Yuasa, Makoto
N1 - Funding Information:
This research was partly supported by the Joint Usage/Research Program of the Photocatalysis International Research Center, Research Institute for Science and Technology, Tokyo University of Science .
PY - 2021/3
Y1 - 2021/3
N2 - The optimal conditions for fabricating a screen-printed diamond electrode for the sensitive detection of L-cysteine (Cys), a non-essential amino acid, were investigated. As-grown (AG-), hydrogen-terminated (H-) and oxygen-terminated boron-doped diamond powders (O-BDDP) were prepared, and BDDP-printed electrodes were fabricated using BDDP-containing inks. Comparing the linear sweep voltammograms of Cys at AG-BDDP-, H-BDDP-, and O-BDDP-printed electrodes, among the three samples, the H-BDDP-printed electrode was found to be the most suitable for the sensitive detection of Cys at low concentrations, showing a steep slope in its calibration curve and a low background current density. In addition, the H-BDDP-printed electrode exhibited a lower limit of detection (0.620 μM) and a more negative oxidation peak potential (+0.663 V vs. Ag/AgCl) and wider linear concentration range (1–194 μM) than a H-boron-doped diamond (BDD) thin-film electrode. It is thought that a slight amount of sp2 carbon on the BDDP contributed to these optimal properties. Using the H-BDDP-printed electrode, Cys was detected in a solution containing electroactive interferents (glutathione and methionine) with a recovery of 86–104%. Therefore, it was concluded that the H-BDDP-printed electrode can be used as a highly sensitive and disposable electrochemical sensor for Cys detection.
AB - The optimal conditions for fabricating a screen-printed diamond electrode for the sensitive detection of L-cysteine (Cys), a non-essential amino acid, were investigated. As-grown (AG-), hydrogen-terminated (H-) and oxygen-terminated boron-doped diamond powders (O-BDDP) were prepared, and BDDP-printed electrodes were fabricated using BDDP-containing inks. Comparing the linear sweep voltammograms of Cys at AG-BDDP-, H-BDDP-, and O-BDDP-printed electrodes, among the three samples, the H-BDDP-printed electrode was found to be the most suitable for the sensitive detection of Cys at low concentrations, showing a steep slope in its calibration curve and a low background current density. In addition, the H-BDDP-printed electrode exhibited a lower limit of detection (0.620 μM) and a more negative oxidation peak potential (+0.663 V vs. Ag/AgCl) and wider linear concentration range (1–194 μM) than a H-boron-doped diamond (BDD) thin-film electrode. It is thought that a slight amount of sp2 carbon on the BDDP contributed to these optimal properties. Using the H-BDDP-printed electrode, Cys was detected in a solution containing electroactive interferents (glutathione and methionine) with a recovery of 86–104%. Therefore, it was concluded that the H-BDDP-printed electrode can be used as a highly sensitive and disposable electrochemical sensor for Cys detection.
KW - Boron-doped diamond
KW - Electrochemical detection
KW - L-cysteine
KW - Screen-printed electrode
UR - http://www.scopus.com/inward/record.url?scp=85096360465&partnerID=8YFLogxK
U2 - 10.1016/j.carbon.2020.10.096
DO - 10.1016/j.carbon.2020.10.096
M3 - Article
AN - SCOPUS:85096360465
VL - 173
SP - 395
EP - 402
JO - Carbon
JF - Carbon
SN - 0008-6223
ER -