Role of PU.1 Expression as an Inflammatory Marker in Experimental Autoimmune Uveoretinitis

Akihiko Umazume; Takeshi Kezuka; Ryusaku Matsuda; Yoshihiko Usui; Hiroki Takahashi; Naoyuki Yamakawa; Takuya Yashiro; Chiharu Nishiyama; Hiroshi Goto

doi:10.1080/09273948.2017.1299867

Role of PU.1 Expression as an Inflammatory Marker in Experimental Autoimmune Uveoretinitis

Akihiko Umazume, Takeshi Kezuka, Ryusaku Matsuda, Yoshihiko Usui, Hiroki Takahashi, Naoyuki Yamakawa, Takuya Yashiro, Chiharu Nishiyama, Hiroshi Goto

Department of Biological Science and Technology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Purpose: PU.1 is an Ets family transcription factor, which is essential for the development of immune system through generation of myeloid and lymphoid lineages. In this study, we investigated PU.1 expression in the retina of mice with experimental autoimmune uveoretinitis (EAU) and the association between PU.1 expression level and inflammation in EAU. Methods: IRBP 1–20 peptide-immunized mice were used. Quantitative PCR, ELISA analysis, cytometric bead array (CBA), assay and immunostaining were conducted using ocular tissues and lymph nodes. Results: Quantitative PCR showed significant increases in mRNA levels of PU.1 in the retina at the peak of inflammation. Immunostaining of retina flat mounts revealed that most PU.1-positive cells were co-stained with anti-CD11c and anti-F4/80 antibodies. PU.1 knockdown in lymph node cells significantly suppressed IRBP-stimulated IFN-γ production measured by ELISA and IL-2 production measured by CBA. Conclusion: PU.1 may play crucial roles in the development and progression of inflammation in EAU.

Original language	English
Pages (from-to)	951-963
Number of pages	13
Journal	Ocular Immunology and Inflammation
Volume	26
Issue number	6
DOIs	https://doi.org/10.1080/09273948.2017.1299867
Publication status	Published - 18 Aug 2018

Keywords

Antigen-specific inflammation
IFN-γ
IL-17
PU.1
experimental autoimmune uveitis

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10.1080/09273948.2017.1299867

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title = "Role of PU.1 Expression as an Inflammatory Marker in Experimental Autoimmune Uveoretinitis",

abstract = "Purpose: PU.1 is an Ets family transcription factor, which is essential for the development of immune system through generation of myeloid and lymphoid lineages. In this study, we investigated PU.1 expression in the retina of mice with experimental autoimmune uveoretinitis (EAU) and the association between PU.1 expression level and inflammation in EAU. Methods: IRBP 1–20 peptide-immunized mice were used. Quantitative PCR, ELISA analysis, cytometric bead array (CBA), assay and immunostaining were conducted using ocular tissues and lymph nodes. Results: Quantitative PCR showed significant increases in mRNA levels of PU.1 in the retina at the peak of inflammation. Immunostaining of retina flat mounts revealed that most PU.1-positive cells were co-stained with anti-CD11c and anti-F4/80 antibodies. PU.1 knockdown in lymph node cells significantly suppressed IRBP-stimulated IFN-γ production measured by ELISA and IL-2 production measured by CBA. Conclusion: PU.1 may play crucial roles in the development and progression of inflammation in EAU.",

keywords = "Antigen-specific inflammation, IFN-γ, IL-17, PU.1, experimental autoimmune uveitis",

author = "Akihiko Umazume and Takeshi Kezuka and Ryusaku Matsuda and Yoshihiko Usui and Hiroki Takahashi and Naoyuki Yamakawa and Takuya Yashiro and Chiharu Nishiyama and Hiroshi Goto",

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year = "2018",

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doi = "10.1080/09273948.2017.1299867",

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T1 - Role of PU.1 Expression as an Inflammatory Marker in Experimental Autoimmune Uveoretinitis

AU - Umazume, Akihiko

AU - Kezuka, Takeshi

AU - Matsuda, Ryusaku

AU - Usui, Yoshihiko

AU - Takahashi, Hiroki

AU - Yamakawa, Naoyuki

AU - Yashiro, Takuya

AU - Nishiyama, Chiharu

AU - Goto, Hiroshi

PY - 2018/8/18

Y1 - 2018/8/18

N2 - Purpose: PU.1 is an Ets family transcription factor, which is essential for the development of immune system through generation of myeloid and lymphoid lineages. In this study, we investigated PU.1 expression in the retina of mice with experimental autoimmune uveoretinitis (EAU) and the association between PU.1 expression level and inflammation in EAU. Methods: IRBP 1–20 peptide-immunized mice were used. Quantitative PCR, ELISA analysis, cytometric bead array (CBA), assay and immunostaining were conducted using ocular tissues and lymph nodes. Results: Quantitative PCR showed significant increases in mRNA levels of PU.1 in the retina at the peak of inflammation. Immunostaining of retina flat mounts revealed that most PU.1-positive cells were co-stained with anti-CD11c and anti-F4/80 antibodies. PU.1 knockdown in lymph node cells significantly suppressed IRBP-stimulated IFN-γ production measured by ELISA and IL-2 production measured by CBA. Conclusion: PU.1 may play crucial roles in the development and progression of inflammation in EAU.

AB - Purpose: PU.1 is an Ets family transcription factor, which is essential for the development of immune system through generation of myeloid and lymphoid lineages. In this study, we investigated PU.1 expression in the retina of mice with experimental autoimmune uveoretinitis (EAU) and the association between PU.1 expression level and inflammation in EAU. Methods: IRBP 1–20 peptide-immunized mice were used. Quantitative PCR, ELISA analysis, cytometric bead array (CBA), assay and immunostaining were conducted using ocular tissues and lymph nodes. Results: Quantitative PCR showed significant increases in mRNA levels of PU.1 in the retina at the peak of inflammation. Immunostaining of retina flat mounts revealed that most PU.1-positive cells were co-stained with anti-CD11c and anti-F4/80 antibodies. PU.1 knockdown in lymph node cells significantly suppressed IRBP-stimulated IFN-γ production measured by ELISA and IL-2 production measured by CBA. Conclusion: PU.1 may play crucial roles in the development and progression of inflammation in EAU.

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KW - experimental autoimmune uveitis

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Role of PU.1 Expression as an Inflammatory Marker in Experimental Autoimmune Uveoretinitis

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