TY - JOUR
T1 - Regulation of human FcERI β chain gene expression by Oct-1
AU - Akizawa, Yushiro
AU - Nishiyama, Chiharu
AU - Hasegawa, Masanari
AU - Maeda, Keiko
AU - Nakahata, Tatsutoshi
AU - Okumura, Ko
AU - Ra, Chisei
AU - Ogawa, Hideoki
N1 - Funding Information:
We thank Drs H. Nakano (Department of Immunology, Juntendo University School of Medicine) and J. H. Butterfield (Mayo Clinic, Rochester) for providing pCR-2F and HMC-1 respectively. We are grateful to Dr M. Nishiyama (Biotechnology Research Center, The University of Tokyo) for his advice in completing this manuscript. We thank members of the Atopy (Allergy) Research Center and Department of Immunology for helpful discussion. We thank Ms T. Tokura for technical assistance, and Ms M. Matsumoto and Ms E. Kawasaki for excellent secretarial assistance. This work was supported in part by a Grant-in-Aid for Young Scientists from The Ministry of Education, Culture, Sports, Science and Technology of Japan (to C. N.).
PY - 2003/5/1
Y1 - 2003/5/1
N2 - The β chain, a component of the high-affinity receptor for IgE (FcERI), plays an important role in IgE-mediated allergic reaction. The β chain accelerates the function of FcERI by amplification of its surface expression and of signal transduction in effector cells such as mast cells and basophils. Two regulatory regions, +60/+66 and +70/+76, for the human β chain gene that are required for the cell-type-specific transcriptional activation were identified by transient reporter assay. Electrophoretic mobility shift assay demonstrated that Oct-1 binds both the regions, among which the +70/+76 Oct-1 motif was critical for the transactivation of the β promoter responsive to Oct-1 overexpression. Regulation of β chain gene expression is discussed.
AB - The β chain, a component of the high-affinity receptor for IgE (FcERI), plays an important role in IgE-mediated allergic reaction. The β chain accelerates the function of FcERI by amplification of its surface expression and of signal transduction in effector cells such as mast cells and basophils. Two regulatory regions, +60/+66 and +70/+76, for the human β chain gene that are required for the cell-type-specific transcriptional activation were identified by transient reporter assay. Electrophoretic mobility shift assay demonstrated that Oct-1 binds both the regions, among which the +70/+76 Oct-1 motif was critical for the transactivation of the β promoter responsive to Oct-1 overexpression. Regulation of β chain gene expression is discussed.
KW - Allergy
KW - IgE receptor
KW - Transcription factors
UR - http://www.scopus.com/inward/record.url?scp=0037408161&partnerID=8YFLogxK
U2 - 10.1093/intimm/dxg055
DO - 10.1093/intimm/dxg055
M3 - Article
C2 - 12697656
AN - SCOPUS:0037408161
SN - 0953-8178
VL - 15
SP - 549
EP - 556
JO - International immunology
JF - International immunology
IS - 5
ER -