Rate control of cell sheet recovery by incorporating hydrophilic pattern in thermoresponsive cell culture dish

Yoshikazu Kumashiro, Teruyuki Matsunaga, Megumi Muraoka, Nobuyuki Tanaka, Kazuyoshi Itoga, Jun Kobayashi, Yumiko Tomiyama, Masatoshi Kuroda, Tatsuya Shimizu, Iwao Hashimoto, Kazuo Umemura, Masayuki Yamato, Teruo Okano

Research output: Contribution to journalArticle

15 Citations (Scopus)


Thready stripe-polyacrylamide (PAAm) pattern was fabricated on a thermoresponsive poly(N-isopropylacrylamide) (PIPAAm) surface, and their surface properties were characterized. A PIPAAm surface spin-coated with positive photoresist was irradiated through a 5 μm/5 μm or a 10 μm/10-μm black and white striped photomask, resulting in the radical polymerization of AAm on the photoirradiated area. After staining with Alexa488 bovine serum albumin, the stripe-patterned surface was clearly observed and the patterned surface was also observed by a phase contrast image of an atomic force microscope. NIH-3T3 (3T3) single cells were able to be cultured at 37°C on the patterned surfaces as well as on a PIPAAm surface without pattern, and the detachment of adhered cells was more rapidly from the patterned surface after reducing temperature. Furthermore, the rate of detachment of 3T3 confluent cell sheet on the patterned surface was accelerated, compared with on a conventional PIPAAm surface under the static condition. The rate control of cell sheet recovery should contribute the preservations of cell phenotype and biological functions of cell sheet for applying to clinical trials.

Original languageEnglish
Pages (from-to)2849-2856
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Issue number8
Publication statusPublished - Aug 2014


  • cell adhesion
  • cell detachment
  • cell sheet
  • micropatterning
  • thermoresponsive surface

Cite this

Kumashiro, Y., Matsunaga, T., Muraoka, M., Tanaka, N., Itoga, K., Kobayashi, J., Tomiyama, Y., Kuroda, M., Shimizu, T., Hashimoto, I., Umemura, K., Yamato, M., & Okano, T. (2014). Rate control of cell sheet recovery by incorporating hydrophilic pattern in thermoresponsive cell culture dish. Journal of Biomedical Materials Research - Part A, 102(8), 2849-2856. https://doi.org/10.1002/jbm.a.34959