Rapid and simple measurement of human C5a-des-Arg level in plasma or serum using monoclonal antibodies

Junji Takeda, Taroh Kinoshita, Yuko Takata, Haruo Kozono, Etsuko Tanaka, Kyongsu Hong, Kozo Inoue

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A new sandwich immunoassay method for measuring human C5a-des-Arg was developed using monoclonal antibody specifically reactive with C5a-des-Arg. Monoclonal antibodies were obtained from a panel of hybridomas produced by fusion of mouse myeloma cells, P3 × 63-AG8,653, with spleen cells from a CBF1(C57BL/6 × BALB/c) mouse immunized with purified C5a. The reactivities of these monoclonal antibodies against C5a, C5a-des-Arg and C5 were tested by solid-phase radioimmunoassay. One of the antibodies reacted with C5a-des-Arg, but not with C5a and C5. By use of this antibody for capturing antibody in sandwich immunoassay, a rapid and simple method was developed for measuring C5a-des-Arg without previous removal of C5. The sensitivity of this assay system was approximately 1 ng/ml for C5a-des-Arg.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalJournal of Immunological Methods
Volume101
Issue number2
DOIs
Publication statusPublished - 3 Aug 1987

Keywords

  • C5a
  • C5a-des-Arg
  • Monoclonal antibody
  • Sandwich immunoassay

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