Purification of L Cell Interferon

Y. Kawade, J. Fujisawa, S. Yonehara, Y. Iwakura, Y. Yamamoto

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Abstract

This chapter describes various processes used for concentrating and purifying L cell interferon, starting from the crude material that consists of serum-free culture fluid of Newcastle disease virus (NDV)-infected L cells. In the final part, a simple procedure is described to purify radiolabeled interferon (biosynthetic label) by immune precipitation. For successful purification, it is essential to start with a large amount of crude material in terms of biological activity. This is mainly for two reasons, both related to the very high specific activity of pure interferon: (a) to keep the protein concentration of interferon samples high enough during purification processes to avoid loss of activity, which rapidly takes place in dilute solutions and (b) to make it possible to monitor the progress of purification and to analyze the purity of the preparations with conventional methods of detecting protein.

Original languageEnglish
Pages (from-to)522-535
Number of pages14
JournalMethods in Enzymology
Volume78
Issue numberC
DOIs
Publication statusPublished - 1 Jan 1981

Cite this

Kawade, Y., Fujisawa, J., Yonehara, S., Iwakura, Y., & Yamamoto, Y. (1981). Purification of L Cell Interferon. Methods in Enzymology, 78(C), 522-535. https://doi.org/10.1016/0076-6879(81)78164-3