PUB4, a CERK1-Interacting Ubiquitin Ligase, Positively Regulates MAMP-Triggered Immunity in Arabidopsis

Yoshitake Desaki, Shohei Takahashi, Kenta Sato, Kanako Maeda, Saki Matsui, Ikuya Yoshimi, Takaki Miura, Jun Ichi Jumonji, Jun Takeda, Kohei Yashima, Masaki Kohari, Takayoshi Suenaga, Hayato Terada, Tomoko Narisawa, Takeo Shimizu, Emi Yumoto, Koji Miyamoto, Mari Narusaka, Yoshihiro Narusaka, Hanae KakuNaoto Shibuya

Research output: Contribution to journalArticle

Abstract

Lysin motif (LysM) receptor-like kinase CERK1 is a co-receptor essential for plant immune responses against carbohydrate microbe-associated molecular patterns (MAMPs). Concerning the immediate downstream signaling components of CERK1, receptor-like cytoplasmic kinases such as PBL27 and other RLCK VII members have been reported to regulate immune responses positively. In this study, we report that a novel CERK1-interacting E3 ubiquitin ligase, PUB4, is also involved in the regulation of MAMP-triggered immune responses. Knockout of PUB4 resulted in the alteration of chitin-induced defense responses, indicating that PUB4 positively regulates reactive oxygen species generation and callose deposition but negatively regulates MAPK activation and defense gene expression. On the other hand, detailed analyses of a double knockout mutant of pub4 and sid2, a mutant of salicylic acid (SA) synthesis pathway, showed that the contradictory phenotype of the pub4 mutant was actually caused by abnormal accumulation of SA in this mutant and that PUB4 is a positive regulator of immune responses. The present and recent findings on the role of PUB4 indicate that PUB4 is a unique E3 ubiquitin ligase involved in the regulation of both plant immunity and growth/development.

Original languageEnglish
Pages (from-to)2573-2583
Number of pages11
JournalPlant and Cell Physiology
Volume60
Issue number11
DOIs
Publication statusPublished - 1 Nov 2019

Fingerprint

Plant Immunity
Ubiquitin-Protein Ligases
Salicylic Acid
Ligases
ubiquitin
Ubiquitin
ligases
Arabidopsis
Immunity
Phosphotransferases
immunity
immune response
microorganisms
ubiquitin-protein ligase
Chitin
Cytoplasmic and Nuclear Receptors
Growth and Development
salicylic acid
mutants
receptors

Keywords

  • CERK1
  • Chitin
  • E3 ligase
  • Immune signaling
  • MAMPs
  • PAMPs
  • PUB

Cite this

Desaki, Yoshitake ; Takahashi, Shohei ; Sato, Kenta ; Maeda, Kanako ; Matsui, Saki ; Yoshimi, Ikuya ; Miura, Takaki ; Jumonji, Jun Ichi ; Takeda, Jun ; Yashima, Kohei ; Kohari, Masaki ; Suenaga, Takayoshi ; Terada, Hayato ; Narisawa, Tomoko ; Shimizu, Takeo ; Yumoto, Emi ; Miyamoto, Koji ; Narusaka, Mari ; Narusaka, Yoshihiro ; Kaku, Hanae ; Shibuya, Naoto. / PUB4, a CERK1-Interacting Ubiquitin Ligase, Positively Regulates MAMP-Triggered Immunity in Arabidopsis. In: Plant and Cell Physiology. 2019 ; Vol. 60, No. 11. pp. 2573-2583.
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abstract = "Lysin motif (LysM) receptor-like kinase CERK1 is a co-receptor essential for plant immune responses against carbohydrate microbe-associated molecular patterns (MAMPs). Concerning the immediate downstream signaling components of CERK1, receptor-like cytoplasmic kinases such as PBL27 and other RLCK VII members have been reported to regulate immune responses positively. In this study, we report that a novel CERK1-interacting E3 ubiquitin ligase, PUB4, is also involved in the regulation of MAMP-triggered immune responses. Knockout of PUB4 resulted in the alteration of chitin-induced defense responses, indicating that PUB4 positively regulates reactive oxygen species generation and callose deposition but negatively regulates MAPK activation and defense gene expression. On the other hand, detailed analyses of a double knockout mutant of pub4 and sid2, a mutant of salicylic acid (SA) synthesis pathway, showed that the contradictory phenotype of the pub4 mutant was actually caused by abnormal accumulation of SA in this mutant and that PUB4 is a positive regulator of immune responses. The present and recent findings on the role of PUB4 indicate that PUB4 is a unique E3 ubiquitin ligase involved in the regulation of both plant immunity and growth/development.",
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Desaki, Y, Takahashi, S, Sato, K, Maeda, K, Matsui, S, Yoshimi, I, Miura, T, Jumonji, JI, Takeda, J, Yashima, K, Kohari, M, Suenaga, T, Terada, H, Narisawa, T, Shimizu, T, Yumoto, E, Miyamoto, K, Narusaka, M, Narusaka, Y, Kaku, H & Shibuya, N 2019, 'PUB4, a CERK1-Interacting Ubiquitin Ligase, Positively Regulates MAMP-Triggered Immunity in Arabidopsis', Plant and Cell Physiology, vol. 60, no. 11, pp. 2573-2583. https://doi.org/10.1093/pcp/pcz151

PUB4, a CERK1-Interacting Ubiquitin Ligase, Positively Regulates MAMP-Triggered Immunity in Arabidopsis. / Desaki, Yoshitake; Takahashi, Shohei; Sato, Kenta; Maeda, Kanako; Matsui, Saki; Yoshimi, Ikuya; Miura, Takaki; Jumonji, Jun Ichi; Takeda, Jun; Yashima, Kohei; Kohari, Masaki; Suenaga, Takayoshi; Terada, Hayato; Narisawa, Tomoko; Shimizu, Takeo; Yumoto, Emi; Miyamoto, Koji; Narusaka, Mari; Narusaka, Yoshihiro; Kaku, Hanae; Shibuya, Naoto.

In: Plant and Cell Physiology, Vol. 60, No. 11, 01.11.2019, p. 2573-2583.

Research output: Contribution to journalArticle

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T1 - PUB4, a CERK1-Interacting Ubiquitin Ligase, Positively Regulates MAMP-Triggered Immunity in Arabidopsis

AU - Desaki, Yoshitake

AU - Takahashi, Shohei

AU - Sato, Kenta

AU - Maeda, Kanako

AU - Matsui, Saki

AU - Yoshimi, Ikuya

AU - Miura, Takaki

AU - Jumonji, Jun Ichi

AU - Takeda, Jun

AU - Yashima, Kohei

AU - Kohari, Masaki

AU - Suenaga, Takayoshi

AU - Terada, Hayato

AU - Narisawa, Tomoko

AU - Shimizu, Takeo

AU - Yumoto, Emi

AU - Miyamoto, Koji

AU - Narusaka, Mari

AU - Narusaka, Yoshihiro

AU - Kaku, Hanae

AU - Shibuya, Naoto

PY - 2019/11/1

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N2 - Lysin motif (LysM) receptor-like kinase CERK1 is a co-receptor essential for plant immune responses against carbohydrate microbe-associated molecular patterns (MAMPs). Concerning the immediate downstream signaling components of CERK1, receptor-like cytoplasmic kinases such as PBL27 and other RLCK VII members have been reported to regulate immune responses positively. In this study, we report that a novel CERK1-interacting E3 ubiquitin ligase, PUB4, is also involved in the regulation of MAMP-triggered immune responses. Knockout of PUB4 resulted in the alteration of chitin-induced defense responses, indicating that PUB4 positively regulates reactive oxygen species generation and callose deposition but negatively regulates MAPK activation and defense gene expression. On the other hand, detailed analyses of a double knockout mutant of pub4 and sid2, a mutant of salicylic acid (SA) synthesis pathway, showed that the contradictory phenotype of the pub4 mutant was actually caused by abnormal accumulation of SA in this mutant and that PUB4 is a positive regulator of immune responses. The present and recent findings on the role of PUB4 indicate that PUB4 is a unique E3 ubiquitin ligase involved in the regulation of both plant immunity and growth/development.

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