Positive Crosstalk of MAMP Signaling Pathways in Rice Cells

Yoshitake Desaki, Ippei Otomo, Daijiro Kobayashi, Yusuke Jikumaru, Yuji Kamiya, Balakrishnan Venkatesh, Shinji Tsuyumu, Hanae Kaku, Naoto Shibuya

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14 Citations (Scopus)

Abstract

Plants have evolved efficient defense mechanisms known as priming and synergy, both of which can mobilize defense responses more extensively against successive pathogen invasion or simultaneous stimulation by different signal molecules. However, the mechanisms underlying these phenomena were largely unknown. In the present study, we used cultured rice cells and combination of purified MAMP molecules as a model system to study the mechanisms of these phenomena. We found that the pretreatment of rice cells with a low concentration of bacterial lipopolysaccharide (LPS) apparently primed the defense responses induced by successive N-acetylchitooctaose (GN8) treatment. On the other hand, simultaneous treatment with GN8 and LPS also resulted in the similar enhancement of defense responses observed for the LPS-induced priming, indicating that the synergistic effects of these MAMPs are basically responsible for such enhancement of defense responses, though the effect could be interpreted as "priming" under some experimental conditions. These results also indicate that such a positive crosstalk of signaling cascade downstream of MAMP receptors seems to occur very rapidly, probably at early step(s) of signaling pathway. Comprehensive analysis of phytohormones revealed a specific enhancement of the synthesis of jasmonic acid (JA), both in the LPS pretreatment and also simultaneous treatment, indicating a role of JA in the enhancement of downstream responses.

Original languageEnglish
Article numbere51953
JournalPloS one
Volume7
Issue number12
DOIs
Publication statusPublished - 14 Dec 2012

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    Desaki, Y., Otomo, I., Kobayashi, D., Jikumaru, Y., Kamiya, Y., Venkatesh, B., Tsuyumu, S., Kaku, H., & Shibuya, N. (2012). Positive Crosstalk of MAMP Signaling Pathways in Rice Cells. PloS one, 7(12), [e51953]. https://doi.org/10.1371/journal.pone.0051953