TY - JOUR
T1 - Polyamines and jasmonic acid induce plasma membrane potential variations in lima bean
AU - Ozawa, Rika
AU - Bertea, Cinzia M.
AU - Foti, Maria
AU - Narayana, Ravishankar
AU - Arimura, Gen Ichiro
AU - Muroi, Atsushi
AU - Maffei, Massimo E.
AU - Takabayashi, Junji
N1 - Funding Information:
This work was supported by: Grant-in-Aid for Scientific Research (S) (No. 19101009 to J.T.); Global COE Program A06 of Kyoto University; CEBIOVEM (Centre of Excellence for Plant and Microbial Biosensing of the University of Turin to M.E.M.).
PY - 2010
Y1 - 2010
N2 - Exogenous polyamines [cadaverine (Cad), putrescine (Put), spermidine (Spd) and spermine (Spm)] elicit the production of volatiles in Lima bean (Phaseolus lunatus). Among the tested PAs, Spm induces the production of some volatile terpenoids that are known to be induced by the spider mite Tetranychus urticae. Spm treatment elicits the biosynthesis of Jasmonic acid (JA), a phytohormone known to regulate the production of the volatile terpenoids. The treatment with JA together with Spm resulted in the increased volatile emission, and predatory mites Phytoseiulus persimilis preferred JA and Spm-treated leaves over those treated with JA alone.5 JA and Spm treatment has no effects on polyamine oxidase (PAO) and Cu-amine oxidase (CuAO) but has a significant induction of calcium influx, ROS production, enzyme activities for NADPH-oxidase complex, superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase and glutathione peroxidase, and gene expressions except for NADPHoxidase complex.5 Here, we report that a plasma membrane potential (Vm) depolarization was observed after polyamine perfusion with an increasing trend: Spm, Cad, Put and Spd. JA perfusion did not alter Vm but the perfusion of JA and the polyamines significantly increased Cad and Put Vm depolarization. When JA was perfused with polyamines, a negative correlation was found between Vm depolarization and the number of amino group of the polyamines tested.
AB - Exogenous polyamines [cadaverine (Cad), putrescine (Put), spermidine (Spd) and spermine (Spm)] elicit the production of volatiles in Lima bean (Phaseolus lunatus). Among the tested PAs, Spm induces the production of some volatile terpenoids that are known to be induced by the spider mite Tetranychus urticae. Spm treatment elicits the biosynthesis of Jasmonic acid (JA), a phytohormone known to regulate the production of the volatile terpenoids. The treatment with JA together with Spm resulted in the increased volatile emission, and predatory mites Phytoseiulus persimilis preferred JA and Spm-treated leaves over those treated with JA alone.5 JA and Spm treatment has no effects on polyamine oxidase (PAO) and Cu-amine oxidase (CuAO) but has a significant induction of calcium influx, ROS production, enzyme activities for NADPH-oxidase complex, superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase and glutathione peroxidase, and gene expressions except for NADPHoxidase complex.5 Here, we report that a plasma membrane potential (Vm) depolarization was observed after polyamine perfusion with an increasing trend: Spm, Cad, Put and Spd. JA perfusion did not alter Vm but the perfusion of JA and the polyamines significantly increased Cad and Put Vm depolarization. When JA was perfused with polyamines, a negative correlation was found between Vm depolarization and the number of amino group of the polyamines tested.
KW - Calcium and ros signalling
KW - Herbivore-induced volatile organic compounds
KW - Jasmonic acid
KW - Lima bean
KW - Polyamines
KW - Quantitative gene expression
KW - Transmembrane potential
UR - http://www.scopus.com/inward/record.url?scp=77954960547&partnerID=8YFLogxK
U2 - 10.4161/psb.5.3.10848
DO - 10.4161/psb.5.3.10848
M3 - Article
C2 - 20200489
AN - SCOPUS:77954960547
SN - 1559-2316
VL - 5
SP - 308
EP - 310
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
IS - 3
ER -