TY - JOUR
T1 - Overproduction of PU.1 in mast cell progenitors
T2 - Its effect on monocyte- and mast cell-specific gene expression
AU - Nishiyama, Chiharu
AU - Nishiyama, Makoto
AU - Ito, Tomonobu
AU - Masaki, Shigehiro
AU - Maeda, Keiko
AU - Masuoka, Nobutaka
AU - Yamane, Hisakazu
AU - Kitamura, Toshio
AU - Ogawa, Hideoki
AU - Okumura, Ko
N1 - Funding Information:
We thank Dr. M. Yoshida for electron microscopy, Dr. K. Yokomizo for May-Grünwald-Giemsa staining, Dr. H. Nakano for providing pCR-2F, and members of Atopy (Allergy) Research Center and Department of Immunology for their helpful discussions, especially for Drs. H. Yagita, A. Nakao, H. Ushio, H. Akiba, Y. Furumoto, M. Nakayama, and T. Yamazaki. We are also grateful for Dr. K. Inaba (Kyoto University) for helpful comments and discussion on the manuscript. We thank Ms. T. Tokura for technical assistance and Ms. M. Matsumoto and Ms. E. Kawasaki for secretarial assistance. This work was supported in part by a Grant-in-Aid for Young Scientists from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to C.N.).
PY - 2004/1/16
Y1 - 2004/1/16
N2 - The Ets family transcription factor PU.1 is required for development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Mouse bone marrow-derived hematopoietic progenitor cells are programmed to differentiate into mast cells, when the cells are maintained in the presence of pokeweed mitogen-stimulated spleen-conditioned medium. However, by retroviral introduction of PU.1 cDNA, the progenitor cells expressed MHC class II, CD11b, CD11c, and F4/80, and acquired the ability to stimulate T cells. Furthermore, PU.1-overproducing cells exhibited the morphology, in part, similar to that of monocyte. These results indicate that the mast cell progenitors still have the ability to express monocyte-specific genes by increased expression of PU.1.
AB - The Ets family transcription factor PU.1 is required for development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Mouse bone marrow-derived hematopoietic progenitor cells are programmed to differentiate into mast cells, when the cells are maintained in the presence of pokeweed mitogen-stimulated spleen-conditioned medium. However, by retroviral introduction of PU.1 cDNA, the progenitor cells expressed MHC class II, CD11b, CD11c, and F4/80, and acquired the ability to stimulate T cells. Furthermore, PU.1-overproducing cells exhibited the morphology, in part, similar to that of monocyte. These results indicate that the mast cell progenitors still have the ability to express monocyte-specific genes by increased expression of PU.1.
KW - MHC class II
KW - Mast cells
KW - Monocyte
KW - PU.1
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=9144231818&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2003.11.145
DO - 10.1016/j.bbrc.2003.11.145
M3 - Article
C2 - 14697219
AN - SCOPUS:9144231818
SN - 0006-291X
VL - 313
SP - 516
EP - 521
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -