OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice

Yoshitake Desaki, Yusuke Kouzai, Yusuke Ninomiya, Ryosuke Iwase, Yumi Shimizu, Keito Seko, Antonio Molinaro, Eiichi Minami, Naoto Shibuya, Hanae Kaku, Yoko Nishizawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Plant cell surface receptor-like kinases (RLKs) mediate the signals from microbe-associated molecular patterns (MAMPs) that induce immune responses. Lipopolysaccharide (LPS), the major constituent of the outer membrane of gram-negative bacteria, is a common MAMP perceived by animals and plants; however, the plant receptors/co-receptors are unknown except for LORE, a bulb-type lectin S-domain RLK (B-lectin SD1-RLK) in Arabidopsis. OsCERK1 is a multifunctional RLK in rice that contains lysin motifs (LysMs) and is essential for the perception of chitin, a fungal MAMP, and peptidoglycan, a bacterial MAMP. Here, we analyzed the relevance of OsCERK1 to LPS perception in rice. Using OsCERK1-knockout mutants (oscerk1), we evaluated hydrogen peroxide (H2O2) production and gene expression after LPS treatment. We also examined the LPS response in knockout mutants for the B-lectin SD1-RLK genes in rice and for all LysM-protein genes in Arabidopsis. Compared with wild-type rice cells, LPS responses in oscerk1 cells were mostly diminished. By contrast, rice lines mutated in either of three B-lectin SD1-RLK genes and Arabidopsis lines mutated in the LysM-protein genes responded normally to LPS. From these results, we conclude that OsCERK1 is an LPS receptor/co-receptor and that the LPS perception systems of rice and Arabidopsis are significantly different.

Original languageEnglish
Pages (from-to)1042-1049
Number of pages8
JournalNew Phytologist
Volume217
Issue number3
DOIs
Publication statusPublished - 1 Feb 2018

Fingerprint

lipopolysaccharides
Lipopolysaccharides
Phosphotransferases
immune response
Mitogen Receptors
Arabidopsis
rice
receptors
phosphotransferases (kinases)
CD14 Antigens
lectins
microorganisms
knockout mutants
Galectins
Chitin
Peptidoglycan
Plant Cells
Cell Surface Receptors
Gram-Negative Bacteria
genes

Keywords

  • Arabidopsis thaliana
  • Oryza sativa (rice)
  • OsCERK1
  • chitin
  • immune response
  • lipopolysaccharide (LPS)
  • microbe-associated molecular patterns (MAMPs)

Cite this

Desaki, Y., Kouzai, Y., Ninomiya, Y., Iwase, R., Shimizu, Y., Seko, K., ... Nishizawa, Y. (2018). OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice. New Phytologist, 217(3), 1042-1049. https://doi.org/10.1111/nph.14941
Desaki, Yoshitake ; Kouzai, Yusuke ; Ninomiya, Yusuke ; Iwase, Ryosuke ; Shimizu, Yumi ; Seko, Keito ; Molinaro, Antonio ; Minami, Eiichi ; Shibuya, Naoto ; Kaku, Hanae ; Nishizawa, Yoko. / OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice. In: New Phytologist. 2018 ; Vol. 217, No. 3. pp. 1042-1049.
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Desaki, Y, Kouzai, Y, Ninomiya, Y, Iwase, R, Shimizu, Y, Seko, K, Molinaro, A, Minami, E, Shibuya, N, Kaku, H & Nishizawa, Y 2018, 'OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice', New Phytologist, vol. 217, no. 3, pp. 1042-1049. https://doi.org/10.1111/nph.14941

OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice. / Desaki, Yoshitake; Kouzai, Yusuke; Ninomiya, Yusuke; Iwase, Ryosuke; Shimizu, Yumi; Seko, Keito; Molinaro, Antonio; Minami, Eiichi; Shibuya, Naoto; Kaku, Hanae; Nishizawa, Yoko.

In: New Phytologist, Vol. 217, No. 3, 01.02.2018, p. 1042-1049.

Research output: Contribution to journalArticle

TY - JOUR

T1 - OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice

AU - Desaki, Yoshitake

AU - Kouzai, Yusuke

AU - Ninomiya, Yusuke

AU - Iwase, Ryosuke

AU - Shimizu, Yumi

AU - Seko, Keito

AU - Molinaro, Antonio

AU - Minami, Eiichi

AU - Shibuya, Naoto

AU - Kaku, Hanae

AU - Nishizawa, Yoko

PY - 2018/2/1

Y1 - 2018/2/1

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KW - Arabidopsis thaliana

KW - Oryza sativa (rice)

KW - OsCERK1

KW - chitin

KW - immune response

KW - lipopolysaccharide (LPS)

KW - microbe-associated molecular patterns (MAMPs)

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