Opposite effects of Trichostatin A on activation of mast cells by different stimulants

Qing hui Wang, Chiharu Nishiyama, Nobuhiro Nakano, Shunsuke Kanada, Mutsuko Hara, Nao Kitamura, Naomi Shimokawa, Chang long Lu, Hideoki Ogawa, Ko Okumura

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Mast cells (MCs) are activated upon stimulation via TLRs or FcεRI, contributing to immune protection and/or leading to allergic diseases. In the present study, the effects of Trichostatin A (TSA) on the activation of MCs were analyzed with bone marrow-derived (BM) MCs. TSA increased the transcription and protein secretion of IL-6 in case of LPS-stimulation, in contrast to the suppressive effect on IgE-mediated activation of BMMCs. Chromatin immunoprecipitation assay showed IgE-mediated signaling-specific suppression of transcription factors recruitment to the IL-6 promoter. TSA-treatment inhibited nuclear translocation of NF-κB following IgE-mediated, but not LPS-induced activation in MCs.

Original languageEnglish
Pages (from-to)2315-2320
Number of pages6
JournalFEBS Letters
Volume584
Issue number11
DOIs
Publication statusPublished - Jun 2010

Keywords

  • FcεRI
  • Mast cell
  • Pharmacology
  • Transcription factor
  • Trichostatin A

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