Novel thermoresponsive nanobio interfaces facilitating cell adhesion and proliferation

We previously reported preparation of functionalized thermoresponsive nanobio interfaces immobilized with cell adhesive peptide, RGDS, or growth factor, insulin (INS) for further application in "cell sheet engineering". Combined these knowledge together, we newly prepared thermoresponsive polymergrafted surfaces immobilized with both RGDS and INS to enhance initial cell adhesion and proliferation. RGDS immobilization facilitated initial cell adhesion, while immobilized INS has no influence on cell adhesion. However, INS immobilization induced faster cell proliferation. RGDS-INS co-immobilized surfaces also showed hydrophilic/hydrophobic surface property alterations with temperature, and induced spontaneously detachment of adhered cells from the modified TR surfaces in single cells or viable contiguous cell monolayers. In conclusion, newly designed TR surfaces modified with both RGDS and INS should be powerful tool for rapid cell sheet fabrication for further cell sheet engineering applications.