Normal levels of serum glycoproteins maintained in β-1,4- galactosyltransferase I-knockout mice

Masahiro Kido, Masahide Asano, Yoichiro Iwakura, Masao Ichinose, Kazumasa Miki, Kiyoshi Furukawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The galactose-mediated clearance of serum glycoproteins from the circulation was evaluated using β-1,4-galactosyltransferase (β-1,4-GalT) I- knockout mice. Partial structural study of the oligosaccharides released from mouse serum glycoproteins revealed that 77.4% of the oligosaccharides from β-1,4-GalT I(+/+) mouse contain galactose, while 7.7% of those from β-1,4- GalT I(-/-) mouse were galactosylated. Under the conditions, no significant change in serum protein concentrations was observed between the normal and mutant mice. The results indicate that the hepatic asialoglycoprotein receptor-mediated system is not functioning in the clearance of endogenous serum glycoproteins.

Original languageEnglish
Pages (from-to)75-79
Number of pages5
JournalFEBS Letters
Volume464
Issue number1-2
DOIs
Publication statusPublished - 24 Dec 1999

Keywords

  • Clearance
  • Serum glycoprotein
  • Undergalactosylation

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