Mitigation of channel clogging in a microfluidic device for capturing circulating tumor cells

Tomoki Konishi, Yuki Jingu, Tatsuya Yoshizawa, Masaru Irita, Toshihiro Suzuki, Masanori Hayase

Research output: Contribution to journalArticle

Abstract

Deterministic lateral displacement (DLD) based microfluidic devices have been developed for capturing circulating tumor cells (CTCs) from the peripheral blood. There was frequent and problematic channel clogging around the micro-post array formed on a microchannel of the device. In this study, various agents were dispersed into the blood specimen to avoid clogging. At first, platelet aggregation was considered to be the cause of the clogging, but even plasmin, which was assumed to decompose platelet aggregations, did not show obvious inhibition of the clogging. Then, enzymes used for cell detachment from tissue were examined and decomposition of the clogging residue was observed. Finally, dispersion of deoxyribonuclease into a blood specimen was found to be effective for the inhibition of clogging. The existence of DNA in the clogging residue was also confirmed by propidium iodide (PI) staining, suggesting DNA adhering to the micro-post.

Original languageEnglish
Pages (from-to)109-116
Number of pages8
JournalInternational Journal of Automation Technology
Volume14
Issue number1
DOIs
Publication statusPublished - 1 Jan 2020

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Microfluidics
Tumors
Blood
Cells
Platelets
DNA
Agglomeration
Enzyme inhibition
Microchannels
Enzymes
Tissue
Decomposition

Keywords

  • Circulating tumor cell (CTC)
  • Clogging
  • Deoxyribonuclease (DNase)
  • Deterministic lateral displacement (DLD)
  • DNA

Cite this

Konishi, Tomoki ; Jingu, Yuki ; Yoshizawa, Tatsuya ; Irita, Masaru ; Suzuki, Toshihiro ; Hayase, Masanori. / Mitigation of channel clogging in a microfluidic device for capturing circulating tumor cells. In: International Journal of Automation Technology. 2020 ; Vol. 14, No. 1. pp. 109-116.
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Mitigation of channel clogging in a microfluidic device for capturing circulating tumor cells. / Konishi, Tomoki; Jingu, Yuki; Yoshizawa, Tatsuya; Irita, Masaru; Suzuki, Toshihiro; Hayase, Masanori.

In: International Journal of Automation Technology, Vol. 14, No. 1, 01.01.2020, p. 109-116.

Research output: Contribution to journalArticle

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