Low voltage pulse application to biological cells

Hidenori Otsuka; Saya Okimura; Masako Nagamura; Daisuke Matsukuma; Koichi Kutsuzawa; Naoki Matsuda; Hirotaka Okabe

doi:10.1587/transele.E96.C.348

Low voltage pulse application to biological cells

Hidenori Otsuka, Saya Okimura, Masako Nagamura, Daisuke Matsukuma, Koichi Kutsuzawa, Naoki Matsuda, Hirotaka Okabe

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

As an application of low electric field to biomedical engineering, this paper attempts to study the dose-effect of biological effects caused by msPEF with experiments on HeLa cells. MTT assay was used to trace the cell electroporation and examine cell viability. It is observed that with the increasing electric field intensity and pulse numbers, IRE effects will occur successively.

Original language	English
Pages (from-to)	348-352
Number of pages	5
Journal	IEICE Transactions on Electronics
Volume	E96-C
Issue number	3
DOIs	https://doi.org/10.1587/transele.E96.C.348
Publication status	Published - Mar 2013

Keywords

Cell viability
Layer by layer (LBL)
Low voltage pulse
Pulsed electric fields (PEF)

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10.1587/transele.E96.C.348

Cite this

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abstract = "As an application of low electric field to biomedical engineering, this paper attempts to study the dose-effect of biological effects caused by msPEF with experiments on HeLa cells. MTT assay was used to trace the cell electroporation and examine cell viability. It is observed that with the increasing electric field intensity and pulse numbers, IRE effects will occur successively.",

keywords = "Cell viability, Layer by layer (LBL), Low voltage pulse, Pulsed electric fields (PEF)",

author = "Hidenori Otsuka and Saya Okimura and Masako Nagamura and Daisuke Matsukuma and Koichi Kutsuzawa and Naoki Matsuda and Hirotaka Okabe",

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AU - Otsuka, Hidenori

AU - Okimura, Saya

AU - Nagamura, Masako

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AU - Kutsuzawa, Koichi

AU - Matsuda, Naoki

AU - Okabe, Hirotaka

PY - 2013/3

Y1 - 2013/3

N2 - As an application of low electric field to biomedical engineering, this paper attempts to study the dose-effect of biological effects caused by msPEF with experiments on HeLa cells. MTT assay was used to trace the cell electroporation and examine cell viability. It is observed that with the increasing electric field intensity and pulse numbers, IRE effects will occur successively.

AB - As an application of low electric field to biomedical engineering, this paper attempts to study the dose-effect of biological effects caused by msPEF with experiments on HeLa cells. MTT assay was used to trace the cell electroporation and examine cell viability. It is observed that with the increasing electric field intensity and pulse numbers, IRE effects will occur successively.

KW - Cell viability

KW - Layer by layer (LBL)

KW - Low voltage pulse

KW - Pulsed electric fields (PEF)

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Low voltage pulse application to biological cells

Abstract

Keywords

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