Long-term retention of the specific functions of the hepatocyte spheroids cultured on micropatterned-surface prepared by UV-photolithography

Akihiro Hirano; Takeshi Ikeya; Tohru Shibuya; Hidenori Otsuka; Satoshi Suzuki; Shin Enosawa; Yuichi Yamasaki; Yukio Nagasaki; Kazunori Kataoka

Long-term retention of the specific functions of the hepatocyte spheroids cultured on micropatterned-surface prepared by UV-photolithography

Akihiro Hirano, Takeshi Ikeya, Tohru Shibuya, Hidenori Otsuka, Satoshi Suzuki, Shin Enosawa, Yuichi Yamasaki, Yukio Nagasaki, Kazunori Kataoka

Department of Applied Chemistry

Research output: Contribution to conference › Paper › peer-review

Abstract

Preparation of the substrate for culturing hepatocyte spheroids with arrayed format was achieved by UV-photolithography using poly(vinyl alcohol)-based non-cell adhesive negative-type photoresist. Bovine aortic endothelial cells (BAECs) seeded onto the substrate only adhered to no-photoresist domains. Rat hepatocytes were subsequently seeded onto the micropatterned-BAECs. Almost hepatocytes formed spheroid only onto the micropatterned-BAECs, but some hepatocytes adhered onto AWP region non-specifically with round form. The hepatocytes on AWP were detached from the surface with increasing culture period. Cytochrome P450 3A1 and 3A2 activities were measured with testosterone-6β-hydroxylation assay. The activities were maintained the initial level during 14 day, and at least 70% of initial activity, after day 30 culture.

Original language	English
Pages	2276
Number of pages	1
Publication status	Published - 2005
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 25 May 2005 → 27 May 2005

Conference

Conference	54th SPSJ Annual Meeting 2005
Country/Territory	Japan
City	Yokohama
Period	25/05/05 → 27/05/05

Keywords

Cell-based biosensor
Cytochrome P450
Hepatocyte spheroid
Microfabncation
Photolithography

Cite this

@conference{71d5e3564e894edea52f8557c9644184,

title = "Long-term retention of the specific functions of the hepatocyte spheroids cultured on micropatterned-surface prepared by UV-photolithography",

abstract = "Preparation of the substrate for culturing hepatocyte spheroids with arrayed format was achieved by UV-photolithography using poly(vinyl alcohol)-based non-cell adhesive negative-type photoresist. Bovine aortic endothelial cells (BAECs) seeded onto the substrate only adhered to no-photoresist domains. Rat hepatocytes were subsequently seeded onto the micropatterned-BAECs. Almost hepatocytes formed spheroid only onto the micropatterned-BAECs, but some hepatocytes adhered onto AWP region non-specifically with round form. The hepatocytes on AWP were detached from the surface with increasing culture period. Cytochrome P450 3A1 and 3A2 activities were measured with testosterone-6β-hydroxylation assay. The activities were maintained the initial level during 14 day, and at least 70% of initial activity, after day 30 culture.",

keywords = "Cell-based biosensor, Cytochrome P450, Hepatocyte spheroid, Microfabncation, Photolithography",

author = "Akihiro Hirano and Takeshi Ikeya and Tohru Shibuya and Hidenori Otsuka and Satoshi Suzuki and Shin Enosawa and Yuichi Yamasaki and Yukio Nagasaki and Kazunori Kataoka",

year = "2005",

language = "English",

pages = "2276",

}

TY - CONF

T1 - Long-term retention of the specific functions of the hepatocyte spheroids cultured on micropatterned-surface prepared by UV-photolithography

AU - Hirano, Akihiro

AU - Ikeya, Takeshi

AU - Shibuya, Tohru

AU - Otsuka, Hidenori

AU - Suzuki, Satoshi

AU - Enosawa, Shin

AU - Yamasaki, Yuichi

AU - Nagasaki, Yukio

AU - Kataoka, Kazunori

PY - 2005

Y1 - 2005

N2 - Preparation of the substrate for culturing hepatocyte spheroids with arrayed format was achieved by UV-photolithography using poly(vinyl alcohol)-based non-cell adhesive negative-type photoresist. Bovine aortic endothelial cells (BAECs) seeded onto the substrate only adhered to no-photoresist domains. Rat hepatocytes were subsequently seeded onto the micropatterned-BAECs. Almost hepatocytes formed spheroid only onto the micropatterned-BAECs, but some hepatocytes adhered onto AWP region non-specifically with round form. The hepatocytes on AWP were detached from the surface with increasing culture period. Cytochrome P450 3A1 and 3A2 activities were measured with testosterone-6β-hydroxylation assay. The activities were maintained the initial level during 14 day, and at least 70% of initial activity, after day 30 culture.

AB - Preparation of the substrate for culturing hepatocyte spheroids with arrayed format was achieved by UV-photolithography using poly(vinyl alcohol)-based non-cell adhesive negative-type photoresist. Bovine aortic endothelial cells (BAECs) seeded onto the substrate only adhered to no-photoresist domains. Rat hepatocytes were subsequently seeded onto the micropatterned-BAECs. Almost hepatocytes formed spheroid only onto the micropatterned-BAECs, but some hepatocytes adhered onto AWP region non-specifically with round form. The hepatocytes on AWP were detached from the surface with increasing culture period. Cytochrome P450 3A1 and 3A2 activities were measured with testosterone-6β-hydroxylation assay. The activities were maintained the initial level during 14 day, and at least 70% of initial activity, after day 30 culture.

KW - Cell-based biosensor

KW - Cytochrome P450

KW - Hepatocyte spheroid

KW - Microfabncation

KW - Photolithography

UR - http://www.scopus.com/inward/record.url?scp=33645640229&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645640229

SP - 2276

T2 - 54th SPSJ Annual Meeting 2005

Y2 - 25 May 2005 through 27 May 2005

ER -

Long-term retention of the specific functions of the hepatocyte spheroids cultured on micropatterned-surface prepared by UV-photolithography

Abstract

Conference

Keywords

Other files and links

Fingerprint

Cite this