TY - JOUR
T1 - Intelligent thermoresponsive polymeric stationary phases for aqueous chromatography of biological compounds
AU - Kikuchi, Akihiko
AU - Okano, Teruo
N1 - Funding Information:
Part of the present work on thermoresponsive aqueous chromatography was carried out by collaboration with Professors Hideko Kanazawa and Yoshiazu Matsushima of Kyoritsu College of Pharmacy, Drs Yukio Hasegawa, Yasuro Shinohara, Kimihiro Yoshizako, and Yoshikatsu Akiyama of Amersham Biosciences Co., and Dr Taiji Yakushiji, and Professor Kiyotaka Sakai of Waseda University. Financial support was provided from the Japan Chemical Innovation Institute (JCII) under the New Energy and Industrial Technology Development Organization (NEDO) from fiscal year of 1996–2000 to which the authors would express sincere acknowledgement.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002/7
Y1 - 2002/7
N2 - Sensitive polymers with external physical, chemical, and electrical stimuli are termed as 'intelligent materials' and currently have been used in variety fields of engineering, and medicine. Numerous research papers utilizing stimuli-responsive intelligent materials are found in the literature to date. In this manuscript, the authors described several applications of surfaces and interfaces modified with stimuli-responsive polymers for stimuli-responsive surface property alteration and their application for the separation sciences. The special attention is paid to the temperature responsive polymers, poly(N-isopropylacrylamide) (PIPAAm) and its derivatives as surface modifiers for novel 'green' chromatography in which only aqueous mobile phase was utilized for separation of bioactive compounds. Several factors were investigated and discussed the effects on separation of bioactive compounds; these include the effects of the temperature-responsive hydrophilic/hydrophobic changes, copolymer composition, graft polymer molecular architecture and the incorporation of charged groups. Furthermore, application of PIPAAm-grafted surfaces for affinity separation of proteins will be discussed. The technique has superior characteristics in reducing organic wastes and costs to run chromatographic separation, and thus must be an environmentally friendly separation tool.
AB - Sensitive polymers with external physical, chemical, and electrical stimuli are termed as 'intelligent materials' and currently have been used in variety fields of engineering, and medicine. Numerous research papers utilizing stimuli-responsive intelligent materials are found in the literature to date. In this manuscript, the authors described several applications of surfaces and interfaces modified with stimuli-responsive polymers for stimuli-responsive surface property alteration and their application for the separation sciences. The special attention is paid to the temperature responsive polymers, poly(N-isopropylacrylamide) (PIPAAm) and its derivatives as surface modifiers for novel 'green' chromatography in which only aqueous mobile phase was utilized for separation of bioactive compounds. Several factors were investigated and discussed the effects on separation of bioactive compounds; these include the effects of the temperature-responsive hydrophilic/hydrophobic changes, copolymer composition, graft polymer molecular architecture and the incorporation of charged groups. Furthermore, application of PIPAAm-grafted surfaces for affinity separation of proteins will be discussed. The technique has superior characteristics in reducing organic wastes and costs to run chromatographic separation, and thus must be an environmentally friendly separation tool.
KW - Aqueous mobile phase
KW - Bioactive compounds
KW - Biocompatibility
KW - Charge density alteration
KW - Chromatography
KW - Dye affinity chromatography
KW - Hydrophilic/hydrophobic property alteration
KW - Penetration control
KW - Separation
KW - Stimuli responsive polymer
KW - Surface function
UR - http://www.scopus.com/inward/record.url?scp=0036643904&partnerID=8YFLogxK
U2 - 10.1016/S0079-6700(02)00013-8
DO - 10.1016/S0079-6700(02)00013-8
M3 - Review article
AN - SCOPUS:0036643904
SN - 0079-6700
VL - 27
SP - 1165
EP - 1193
JO - Progress in Polymer Science (Oxford)
JF - Progress in Polymer Science (Oxford)
IS - 6
ER -