Influence of the difference in refractive index on the interface of an object and the surroundings in near-infrared fluorescence tomography

Maho Takematsu, Masakazu Umezawa, Toshihiro Sera, Yuichi Kitagawa, Hiroyuki Kurahashi, Shuta Yamada, Kyohei Okubo, Masao Kamimura, Hideo Yokota, Kohei Soga

Research output: Contribution to journalArticlepeer-review

Abstract

The refraction of fluorescence from the inside of a sample at the surface results in fluctuations in fluorescence computed tomography (CT). We evaluated the influence of the difference in refractive index (RI) between the sample body and the surroundings on fluorescence CT results. The brightest fluorescent point is away from the correct point on the tomograms owing to the refraction. The speculated position is determined as the exact point if the RI ratio ranges between 0.97 and 1.03 by immersing the body in an RI matching liquid. The results can help in experimental settings of fluorescence CT for acquiring three-dimensional positional information.

Original languageEnglish
Pages (from-to)638-644
Number of pages7
JournalApplied Optics
Volume61
Issue number2
DOIs
Publication statusPublished - 10 Jan 2022

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