Immunostimulatory effects of Heyndrickxia coagulans SANK70258

Yuki Ikeda; Niya Yamashita; Naoto Ito; Natsuki Minamikawa; Hotaka Okamura; Takuya Yashiro; Masakazu Hachisu; Masanori Aida; Ryouichi Yamada; Kazuki Nagata; Chiharu Nishiyama

doi:10.1093/bbb/zbae203

Immunostimulatory effects of Heyndrickxia coagulans SANK70258

Yuki Ikeda, Niya Yamashita, Naoto Ito, Natsuki Minamikawa, Hotaka Okamura, Takuya Yashiro, Masakazu Hachisu, Masanori Aida, Ryouichi Yamada, Kazuki Nagata, Chiharu Nishiyama

Research output: Contribution to journal › Article › peer-review

Abstract

Here, we examined the immunomodulating effects of Heyndrickxia coagulans SANK70258 (SANK70258). Mouse splenocytes treated with γ-ray-irradiated SANK70258 produced higher levels of interferon (IFN)-γ than those with 7 types of lactic acid bacteria. IFN-γ was mainly produced by natural killer (NK) cells, involving IL-12/IL-23, dendritic cells (DCs), and NF-_κB signaling. SANK70258 induced the release of IL-6, IL-10, and IL-12p40 from mouse DCs and the expression of cytokine genes in the human monocyte. Cytokine release from SANK70258-treated DCs was partially reduced by the knockdown of Tlr2 or Nod2, and was abolished by Myd88 knockout. DC-stimulating components of SANK70258 were enriched in ether- and butanol-insoluble peptidoglycan-related fractions. The SANK70258 component induced high levels of immunoglobulin (Ig) A production in Peyer's patch cells, and its oral intake significantly increased intestinal IgA and IgA-expressing B cells in Peyer's patches in mice. We conclude that the SANK70258 component exhibits high activity as an immunostimulant that induces the production of IFN-γ and IgA.

Original language	English
Pages (from-to)	622-632
Number of pages	11
Journal	Bioscience, Biotechnology and Biochemistry
Volume	89
Issue number	4
DOIs	https://doi.org/10.1093/bbb/zbae203
Publication status	Published - 1 Apr 2025

Keywords

Heyndrickxia coagulans SANK70258
IFN-γ
IL-10
IgA
dendritic cells

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10.1093/bbb/zbae203

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title = "Immunostimulatory effects of Heyndrickxia coagulans SANK70258",

abstract = "Here, we examined the immunomodulating effects of Heyndrickxia coagulans SANK70258 (SANK70258). Mouse splenocytes treated with γ-ray-irradiated SANK70258 produced higher levels of interferon (IFN)-γ than those with 7 types of lactic acid bacteria. IFN-γ was mainly produced by natural killer (NK) cells, involving IL-12/IL-23, dendritic cells (DCs), and NF-κB signaling. SANK70258 induced the release of IL-6, IL-10, and IL-12p40 from mouse DCs and the expression of cytokine genes in the human monocyte. Cytokine release from SANK70258-treated DCs was partially reduced by the knockdown of Tlr2 or Nod2, and was abolished by Myd88 knockout. DC-stimulating components of SANK70258 were enriched in ether- and butanol-insoluble peptidoglycan-related fractions. The SANK70258 component induced high levels of immunoglobulin (Ig) A production in Peyer's patch cells, and its oral intake significantly increased intestinal IgA and IgA-expressing B cells in Peyer's patches in mice. We conclude that the SANK70258 component exhibits high activity as an immunostimulant that induces the production of IFN-γ and IgA.",

keywords = "Heyndrickxia coagulans SANK70258, IFN-γ, IL-10, IgA, dendritic cells",

author = "Yuki Ikeda and Niya Yamashita and Naoto Ito and Natsuki Minamikawa and Hotaka Okamura and Takuya Yashiro and Masakazu Hachisu and Masanori Aida and Ryouichi Yamada and Kazuki Nagata and Chiharu Nishiyama",

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doi = "10.1093/bbb/zbae203",

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AU - Ikeda, Yuki

AU - Yamashita, Niya

AU - Ito, Naoto

AU - Minamikawa, Natsuki

AU - Okamura, Hotaka

AU - Yashiro, Takuya

AU - Hachisu, Masakazu

AU - Aida, Masanori

AU - Yamada, Ryouichi

AU - Nagata, Kazuki

AU - Nishiyama, Chiharu

PY - 2025/4/1

Y1 - 2025/4/1

N2 - Here, we examined the immunomodulating effects of Heyndrickxia coagulans SANK70258 (SANK70258). Mouse splenocytes treated with γ-ray-irradiated SANK70258 produced higher levels of interferon (IFN)-γ than those with 7 types of lactic acid bacteria. IFN-γ was mainly produced by natural killer (NK) cells, involving IL-12/IL-23, dendritic cells (DCs), and NF-κB signaling. SANK70258 induced the release of IL-6, IL-10, and IL-12p40 from mouse DCs and the expression of cytokine genes in the human monocyte. Cytokine release from SANK70258-treated DCs was partially reduced by the knockdown of Tlr2 or Nod2, and was abolished by Myd88 knockout. DC-stimulating components of SANK70258 were enriched in ether- and butanol-insoluble peptidoglycan-related fractions. The SANK70258 component induced high levels of immunoglobulin (Ig) A production in Peyer's patch cells, and its oral intake significantly increased intestinal IgA and IgA-expressing B cells in Peyer's patches in mice. We conclude that the SANK70258 component exhibits high activity as an immunostimulant that induces the production of IFN-γ and IgA.

AB - Here, we examined the immunomodulating effects of Heyndrickxia coagulans SANK70258 (SANK70258). Mouse splenocytes treated with γ-ray-irradiated SANK70258 produced higher levels of interferon (IFN)-γ than those with 7 types of lactic acid bacteria. IFN-γ was mainly produced by natural killer (NK) cells, involving IL-12/IL-23, dendritic cells (DCs), and NF-κB signaling. SANK70258 induced the release of IL-6, IL-10, and IL-12p40 from mouse DCs and the expression of cytokine genes in the human monocyte. Cytokine release from SANK70258-treated DCs was partially reduced by the knockdown of Tlr2 or Nod2, and was abolished by Myd88 knockout. DC-stimulating components of SANK70258 were enriched in ether- and butanol-insoluble peptidoglycan-related fractions. The SANK70258 component induced high levels of immunoglobulin (Ig) A production in Peyer's patch cells, and its oral intake significantly increased intestinal IgA and IgA-expressing B cells in Peyer's patches in mice. We conclude that the SANK70258 component exhibits high activity as an immunostimulant that induces the production of IFN-γ and IgA.

KW - Heyndrickxia coagulans SANK70258

KW - IFN-γ

KW - IL-10

KW - IgA

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 4

ER -

Immunostimulatory effects of Heyndrickxia coagulans SANK70258

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