Immobilization of RGDS peptides onto temperature-responsive culture dishes by Avidin-Biotin chemistry

Masanori Nishi; Jun Kobayashi; Masayuki Yamato; Akihiko Kikuchi; Katsumi Uchida; Hirofumi Yajima; Teruo Okano

Immobilization of RGDS peptides onto temperature-responsive culture dishes by Avidin-Biotin chemistry

Masanori Nishi, Jun Kobayashi, Masayuki Yamato, Akihiko Kikuchi, Katsumi Uchida, Hirofumi Yajima, Teruo Okano

Department of Materials Science and Technology

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper describes a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(N-isopropylacrylamide-co- 2-carboxyisopropylacrylamide)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were also examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G₁₂RGDS-immobilized thermoresponsive TCPS at 37°C. Moreover, adhered HUVECs were detached after lowering temperature to 20°C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G₁₂RGDS. Consequently, thermoresponsive TCPS modified with G₁₂RGDS peptide allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.

Original language	English
Number of pages	1
Publication status	Published - 19 Oct 2006
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 24 May 2006 → 26 May 2006

Conference

Conference	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	24/05/06 → 26/05/06

Keywords

2-carboxyisopropylacrylamide
Avidin
Biotin
Cell culture
Electron beam
Thermoresponsive surface

Cite this

@conference{b400a583dab04b169447be3afb41695d,

title = "Immobilization of RGDS peptides onto temperature-responsive culture dishes by Avidin-Biotin chemistry",

abstract = "This paper describes a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(N-isopropylacrylamide-co- 2-carboxyisopropylacrylamide)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were also examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G12RGDS-immobilized thermoresponsive TCPS at 37°C. Moreover, adhered HUVECs were detached after lowering temperature to 20°C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G12RGDS. Consequently, thermoresponsive TCPS modified with G12RGDS peptide allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.",

keywords = "2-carboxyisopropylacrylamide, Avidin, Biotin, Cell culture, Electron beam, Thermoresponsive surface",

author = "Masanori Nishi and Jun Kobayashi and Masayuki Yamato and Akihiko Kikuchi and Katsumi Uchida and Hirofumi Yajima and Teruo Okano",

year = "2006",

month = oct,

day = "19",

language = "English",

note = "55th SPSJ Annual Meeting ; Conference date: 24-05-2006 Through 26-05-2006",

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TY - CONF

T1 - Immobilization of RGDS peptides onto temperature-responsive culture dishes by Avidin-Biotin chemistry

AU - Nishi, Masanori

AU - Kobayashi, Jun

AU - Yamato, Masayuki

AU - Kikuchi, Akihiko

AU - Uchida, Katsumi

AU - Yajima, Hirofumi

AU - Okano, Teruo

PY - 2006/10/19

Y1 - 2006/10/19

N2 - This paper describes a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(N-isopropylacrylamide-co- 2-carboxyisopropylacrylamide)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were also examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G12RGDS-immobilized thermoresponsive TCPS at 37°C. Moreover, adhered HUVECs were detached after lowering temperature to 20°C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G12RGDS. Consequently, thermoresponsive TCPS modified with G12RGDS peptide allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.

AB - This paper describes a novel method to immobilize biomolecules such as growth factors and enzymes onto thermoresponsive poly(N-isopropylacrylamide-co- 2-carboxyisopropylacrylamide)-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of RGDS peptides with different spacer length on the behavior of cell attachment / detachment were also examined. Human umbilical vein endothelial cells (HUVECs) adhered well on G12RGDS-immobilized thermoresponsive TCPS at 37°C. Moreover, adhered HUVECs were detached after lowering temperature to 20°C. However, weak adhesion of HUVECs was observed on thermoresponsive TCPS modified with shorter and longer peptides than G12RGDS. Consequently, thermoresponsive TCPS modified with G12RGDS peptide allows cell culture under the serum-free medium and noninvasive cell recovery by lowering temperature.

KW - 2-carboxyisopropylacrylamide

KW - Avidin

KW - Biotin

KW - Cell culture

KW - Electron beam

KW - Thermoresponsive surface

UR - http://www.scopus.com/inward/record.url?scp=33749842375&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33749842375

T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

ER -

Immobilization of RGDS peptides onto temperature-responsive culture dishes by Avidin-Biotin chemistry

Abstract

Conference

Keywords

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