Identification of minimal p53 promoter region regulated by MALAT1 in human lung adenocarcinoma cells

Keiko Tano; Rena Onoguchi-Mizutani; Fouzia Yeasmin; Fumiaki Uchiumi; Yutaka Suzuki; Tetsushi Yada; Nobuyoshi Akimitsu

doi:10.3389/fgene.2017.00208

Identification of minimal p53 promoter region regulated by MALAT1 in human lung adenocarcinoma cells

Keiko Tano, Rena Onoguchi-Mizutani, Fouzia Yeasmin, Fumiaki Uchiumi, Yutaka Suzuki, Tetsushi Yada, Nobuyoshi Akimitsu

Department of Medicinal and Life Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that these upregulations were mediated by transcriptional activation of p53 through MALAT1 depletion. In addition, we identified a minimal MALAT1-responsive region in the P1 promoter of p53 gene. Flow cytometry analysis revealed that MALAT1-depleted cells exhibited G1 cell cycle arrest. These results suggest that MALAT1 affects the expression of p53 target genes through repressing p53 promoter activity, leading to influence the cell cycle progression.

Original language	English
Article number	208
Journal	Frontiers in Genetics
Volume	9
Issue number	MAR
DOIs	https://doi.org/10.3389/fgene.2017.00208
Publication status	Published - 26 Mar 2018

Keywords

Adenocarcinoma
Genetic
Genetic
Noncoding RNA
Promoter regions
TP53
Transcription

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10.3389/fgene.2017.00208

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title = "Identification of minimal p53 promoter region regulated by MALAT1 in human lung adenocarcinoma cells",

abstract = "The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that these upregulations were mediated by transcriptional activation of p53 through MALAT1 depletion. In addition, we identified a minimal MALAT1-responsive region in the P1 promoter of p53 gene. Flow cytometry analysis revealed that MALAT1-depleted cells exhibited G1 cell cycle arrest. These results suggest that MALAT1 affects the expression of p53 target genes through repressing p53 promoter activity, leading to influence the cell cycle progression.",

keywords = "Adenocarcinoma, Genetic, Genetic, Noncoding RNA, Promoter regions, TP53, Transcription",

author = "Keiko Tano and Rena Onoguchi-Mizutani and Fouzia Yeasmin and Fumiaki Uchiumi and Yutaka Suzuki and Tetsushi Yada and Nobuyoshi Akimitsu",

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AU - Tano, Keiko

AU - Onoguchi-Mizutani, Rena

AU - Yeasmin, Fouzia

AU - Uchiumi, Fumiaki

AU - Suzuki, Yutaka

AU - Yada, Tetsushi

AU - Akimitsu, Nobuyoshi

PY - 2018/3/26

Y1 - 2018/3/26

N2 - The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that these upregulations were mediated by transcriptional activation of p53 through MALAT1 depletion. In addition, we identified a minimal MALAT1-responsive region in the P1 promoter of p53 gene. Flow cytometry analysis revealed that MALAT1-depleted cells exhibited G1 cell cycle arrest. These results suggest that MALAT1 affects the expression of p53 target genes through repressing p53 promoter activity, leading to influence the cell cycle progression.

AB - The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that these upregulations were mediated by transcriptional activation of p53 through MALAT1 depletion. In addition, we identified a minimal MALAT1-responsive region in the P1 promoter of p53 gene. Flow cytometry analysis revealed that MALAT1-depleted cells exhibited G1 cell cycle arrest. These results suggest that MALAT1 affects the expression of p53 target genes through repressing p53 promoter activity, leading to influence the cell cycle progression.

KW - Adenocarcinoma

KW - Genetic

KW - Noncoding RNA

KW - Promoter regions

KW - TP53

KW - Transcription

UR - https://www.scopus.com/pages/publications/85044845336

U2 - 10.3389/fgene.2017.00208

DO - 10.3389/fgene.2017.00208

M3 - Article

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VL - 9

JO - Frontiers in Genetics

JF - Frontiers in Genetics

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M1 - 208

ER -

Identification of minimal p53 promoter region regulated by MALAT1 in human lung adenocarcinoma cells

Abstract

Keywords

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