Abstract
The PEG segment becomes tethered like chain on the surface, which caused extremely high dispersion stability of gold nanoparticles even in high salt concentration, acidic and basic conditions. Fluorescein installed-gold nanoparticles were specifically bound to the surface of anti-fluorescein immobilized SPR sensor chip. When low molecular weight fluorescein was analyzed by the FITC-immobilized gold colloid surface, extremely strong signal was observed by the exchange of the gold nanoparticles with the free fluorescein by optimizing the amount of immobilized antibody, binding time of gold nanoparticles, concentration of gold nanoparticles, etc. The non-fouling gold nanoparticles coupled with non-fouling SPR sensor chip is anticipated as a highly sensitive low MW sensor.
| Original language | English |
|---|---|
| Pages | 2280 |
| Number of pages | 1 |
| Publication status | Published - 2005 |
| Event | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 25 May 2005 → 27 May 2005 |
Conference
| Conference | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country/Territory | Japan |
| City | Yokohama |
| Period | 25/05/05 → 27/05/05 |
Keywords
- Biosensor
- Gold nanoparticles
- Low molecular weight analyte
- PEG
- SPR