High capacity lactate biofuel cell using enzyme cascade without nad

Isao Shitanda; Motoki Tsunaga; Hikari Watanabe; Masayuki Itagaki; Seiya Tsujimura; Tsutomu Mikawa

doi:10.1246/cl.210064

High capacity lactate biofuel cell using enzyme cascade without nad

Isao Shitanda, Motoki Tsunaga, Hikari Watanabe, Masayuki Itagaki, Seiya Tsujimura, Tsutomu Mikawa

Department of Pure and Applied Chemistry

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We describe a modification of the lactate biofuel cell to increase its capacity. A new multi-step enzyme reaction system without NAD was prepared using a bioanode modified with lactate oxidase, pyruvate decarboxylase, and pyrroloquinoline quinone-dependent aldehyde dehydrogenase. Its operating time was 6 times longer than that of the biofuel cell using the lactate oxidase-modified bioanode at constant current measurement of 1mAcm 2 because of suppression of the diffusion overpotential due to the decrease in the fuel concentration.

Original language	English
Pages (from-to)	1160-1163
Number of pages	4
Journal	Chemistry Letters
Volume	50
Issue number	6
DOIs	https://doi.org/10.1246/cl.210064
Publication status	Published - Jun 2021

Keywords

Biofuel cell
Enzyme cascade
Lactate

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10.1246/cl.210064

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title = "High capacity lactate biofuel cell using enzyme cascade without nad",

abstract = "We describe a modification of the lactate biofuel cell to increase its capacity. A new multi-step enzyme reaction system without NAD was prepared using a bioanode modified with lactate oxidase, pyruvate decarboxylase, and pyrroloquinoline quinone-dependent aldehyde dehydrogenase. Its operating time was 6 times longer than that of the biofuel cell using the lactate oxidase-modified bioanode at constant current measurement of 1mAcm 2 because of suppression of the diffusion overpotential due to the decrease in the fuel concentration.",

keywords = "Biofuel cell, Enzyme cascade, Lactate",

author = "Isao Shitanda and Motoki Tsunaga and Hikari Watanabe and Masayuki Itagaki and Seiya Tsujimura and Tsutomu Mikawa",

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T1 - High capacity lactate biofuel cell using enzyme cascade without nad

AU - Shitanda, Isao

AU - Tsunaga, Motoki

AU - Watanabe, Hikari

AU - Itagaki, Masayuki

AU - Tsujimura, Seiya

AU - Mikawa, Tsutomu

PY - 2021/6

Y1 - 2021/6

N2 - We describe a modification of the lactate biofuel cell to increase its capacity. A new multi-step enzyme reaction system without NAD was prepared using a bioanode modified with lactate oxidase, pyruvate decarboxylase, and pyrroloquinoline quinone-dependent aldehyde dehydrogenase. Its operating time was 6 times longer than that of the biofuel cell using the lactate oxidase-modified bioanode at constant current measurement of 1mAcm 2 because of suppression of the diffusion overpotential due to the decrease in the fuel concentration.

AB - We describe a modification of the lactate biofuel cell to increase its capacity. A new multi-step enzyme reaction system without NAD was prepared using a bioanode modified with lactate oxidase, pyruvate decarboxylase, and pyrroloquinoline quinone-dependent aldehyde dehydrogenase. Its operating time was 6 times longer than that of the biofuel cell using the lactate oxidase-modified bioanode at constant current measurement of 1mAcm 2 because of suppression of the diffusion overpotential due to the decrease in the fuel concentration.

KW - Biofuel cell

KW - Enzyme cascade

KW - Lactate

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DO - 10.1246/cl.210064

M3 - Article

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SP - 1160

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JO - Chemistry Letters

JF - Chemistry Letters

IS - 6

ER -

High capacity lactate biofuel cell using enzyme cascade without nad

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Keywords

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