Functional analysis of PU.1 domains in monocyte-specific gene regulation

Chiharu Nishiyama; Makoto Nishiyama; Tomonobu Ito; Shigehiro Masaki; Nobutaka Masuoka; Hisakazu Yamane; Toshio Kitamura; Hideoki Ogawa; Ko Okumura

doi:10.1016/S0014-5793(04)00116-4

Functional analysis of PU.1 domains in monocyte-specific gene regulation

Chiharu Nishiyama, Makoto Nishiyama, Tomonobu Ito, Shigehiro Masaki, Nobutaka Masuoka, Hisakazu Yamane, Toshio Kitamura, Hideoki Ogawa, Ko Okumura

Department of Biological Science and Technology

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

The Ets family transcription factor PU.1 is required for the development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Recently we found that overproduction of PU.1 in mouse bone marrow-derived mast cell progenitors induced the expression of monocyte-specific genes. This prompted us to analyze the functions of each domain of PU.1 in monocyte-specific gene expression, using transfection of mast cell progenitors with a series of retrovirus vectors for overexpression of various truncation mutants. Both the acidic region and the Ets domain of PU.1 were required for expression of monocyte-specific genes, and for enhanced interleukin-6 production in response to lipopolysaccharide. The Gln-rich region was suggested to be involved in expression of both MHC class II and F4/80. On the other hand, when PU.1 protein lacking the PEST domain was produced in the progenitor cells, expression of monocyte-specific genes was substantially enhanced, suggesting that the PEST domain plays a negative role in monocyte-specific gene expression.

Original language	English
Pages (from-to)	63-68
Number of pages	6
Journal	FEBS Letters
Volume	561
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(04)00116-4
Publication status	Published - 12 Mar 2004

Keywords

CD11b
CD11c
DC, dendritic cell
F4/80
IRF, interferon regulatory factor
LPS, lipopolysaccharide
MHC class II
PEST
PU.1
TLR, Toll-like receptor

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10.1016/S0014-5793(04)00116-4

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@article{d31321c07b154b92ad03a51354aeacc5,

title = "Functional analysis of PU.1 domains in monocyte-specific gene regulation",

abstract = "The Ets family transcription factor PU.1 is required for the development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Recently we found that overproduction of PU.1 in mouse bone marrow-derived mast cell progenitors induced the expression of monocyte-specific genes. This prompted us to analyze the functions of each domain of PU.1 in monocyte-specific gene expression, using transfection of mast cell progenitors with a series of retrovirus vectors for overexpression of various truncation mutants. Both the acidic region and the Ets domain of PU.1 were required for expression of monocyte-specific genes, and for enhanced interleukin-6 production in response to lipopolysaccharide. The Gln-rich region was suggested to be involved in expression of both MHC class II and F4/80. On the other hand, when PU.1 protein lacking the PEST domain was produced in the progenitor cells, expression of monocyte-specific genes was substantially enhanced, suggesting that the PEST domain plays a negative role in monocyte-specific gene expression.",

keywords = "CD11b, CD11c, DC, dendritic cell, F4/80, IRF, interferon regulatory factor, LPS, lipopolysaccharide, MHC class II, PEST, PU.1, TLR, Toll-like receptor",

author = "Chiharu Nishiyama and Makoto Nishiyama and Tomonobu Ito and Shigehiro Masaki and Nobutaka Masuoka and Hisakazu Yamane and Toshio Kitamura and Hideoki Ogawa and Ko Okumura",

note = "Funding Information: We thank Dr. M. Yoshida for electron microscopy, Dr. K. Yokomizo for May-Gr{\"u}nwald-Giemsa staining, Dr. H. Nakano for providing pCR-2F, and members of the Atopy (Allergy) Research Center and Department of Immunology for their helpful discussions, especially Drs. H. Yagita, A. Nakao, H. Ushio, H. Akiba, Y. Furumoto, M. Nakayama, and T. Yamazaki. We are also grateful for Dr. K. Inaba (Kyoto University) for helpful comments and discussion on the manuscript. We thank Ms. T. Tokura for technical assistance and Ms. M. Matsumoto and Ms. E. Kawasaki for excellent secretarial assistance. This work was supported in part by a Grant-in-Aid for Young Scientists from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to C.N.).",

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day = "12",

doi = "10.1016/S0014-5793(04)00116-4",

language = "English",

volume = "561",

pages = "63--68",

journal = "FEBS Letters",

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T1 - Functional analysis of PU.1 domains in monocyte-specific gene regulation

AU - Nishiyama, Chiharu

AU - Nishiyama, Makoto

AU - Ito, Tomonobu

AU - Masaki, Shigehiro

AU - Masuoka, Nobutaka

AU - Yamane, Hisakazu

AU - Kitamura, Toshio

AU - Ogawa, Hideoki

AU - Okumura, Ko

N1 - Funding Information: We thank Dr. M. Yoshida for electron microscopy, Dr. K. Yokomizo for May-Grünwald-Giemsa staining, Dr. H. Nakano for providing pCR-2F, and members of the Atopy (Allergy) Research Center and Department of Immunology for their helpful discussions, especially Drs. H. Yagita, A. Nakao, H. Ushio, H. Akiba, Y. Furumoto, M. Nakayama, and T. Yamazaki. We are also grateful for Dr. K. Inaba (Kyoto University) for helpful comments and discussion on the manuscript. We thank Ms. T. Tokura for technical assistance and Ms. M. Matsumoto and Ms. E. Kawasaki for excellent secretarial assistance. This work was supported in part by a Grant-in-Aid for Young Scientists from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to C.N.).

PY - 2004/3/12

Y1 - 2004/3/12

N2 - The Ets family transcription factor PU.1 is required for the development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Recently we found that overproduction of PU.1 in mouse bone marrow-derived mast cell progenitors induced the expression of monocyte-specific genes. This prompted us to analyze the functions of each domain of PU.1 in monocyte-specific gene expression, using transfection of mast cell progenitors with a series of retrovirus vectors for overexpression of various truncation mutants. Both the acidic region and the Ets domain of PU.1 were required for expression of monocyte-specific genes, and for enhanced interleukin-6 production in response to lipopolysaccharide. The Gln-rich region was suggested to be involved in expression of both MHC class II and F4/80. On the other hand, when PU.1 protein lacking the PEST domain was produced in the progenitor cells, expression of monocyte-specific genes was substantially enhanced, suggesting that the PEST domain plays a negative role in monocyte-specific gene expression.

AB - The Ets family transcription factor PU.1 is required for the development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Recently we found that overproduction of PU.1 in mouse bone marrow-derived mast cell progenitors induced the expression of monocyte-specific genes. This prompted us to analyze the functions of each domain of PU.1 in monocyte-specific gene expression, using transfection of mast cell progenitors with a series of retrovirus vectors for overexpression of various truncation mutants. Both the acidic region and the Ets domain of PU.1 were required for expression of monocyte-specific genes, and for enhanced interleukin-6 production in response to lipopolysaccharide. The Gln-rich region was suggested to be involved in expression of both MHC class II and F4/80. On the other hand, when PU.1 protein lacking the PEST domain was produced in the progenitor cells, expression of monocyte-specific genes was substantially enhanced, suggesting that the PEST domain plays a negative role in monocyte-specific gene expression.

KW - CD11b

KW - CD11c

KW - DC, dendritic cell

KW - F4/80

KW - IRF, interferon regulatory factor

KW - LPS, lipopolysaccharide

KW - MHC class II

KW - PEST

KW - PU.1

KW - TLR, Toll-like receptor

UR - http://www.scopus.com/inward/record.url?scp=1542316350&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(04)00116-4

DO - 10.1016/S0014-5793(04)00116-4

M3 - Article

C2 - 15013752

AN - SCOPUS:1542316350

SN - 0014-5793

VL - 561

SP - 63

EP - 68

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Functional analysis of PU.1 domains in monocyte-specific gene regulation

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Keywords

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