Fabrication of Storable Surface-Functionalized Power-Free Microfluidic Chip for Sensitive MicroRNA Detection Utilizing Ultraviolet Grafting

Ryo Ishihara, Kanta Tanabe, Shoko Inomata, Ryo Matsui, Ryoichi Kitane, Kazuo Hosokawa, Mizuo Maeda, Akihiko Kikuchi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Recently, circulating microRNAs (miRNAs) in body fluids have been reported as one of the cancer diagnostic biomarkers, and detection of miRNAs is expected to improve early cancer diagnosis. In this study, a surface-functionalized power-free (SF-PF) microchip for miRNA detection was fabricated by applying UV-light-induced graft polymerization of poly(2-aminoethyl methacrylate) to a poly(dimethylsiloxane) power-free microchip and by immobilizing capture probe DNA to the graft chains. The required time, sample volume, and limit of detection of miR-500a-3p were 18 min, 0.5 μL, and 41 fmol/L, respectively. In addition, the SF-PF microchip could be stored at ambient temperature and humidity for at least 1 week.

Original languageEnglish
Pages (from-to)10464-10468
Number of pages5
JournalIndustrial and Engineering Chemistry Research
Volume59
Issue number22
DOIs
Publication statusPublished - 3 Jun 2020

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